The aims of the present research were to develop and characterize 4-hydroxycoumarin-loaded liposomes via the determination of encapsulation efficiency, antioxidant capacity, vesicle size, polydispersity index (PDI), zeta potential, conductivity, mobility, density, surface tension, viscosity, and 60-day storage stability. The encapsulation efficien...

By Aleksandra Jovanović, Edina Avdović, Ana Plećić, Vojislav Ćirić, Branko Bugarski, Zoran Marković

Steppe peony (Paeonia tenuifolia L., Paeoniaceae) is a perennial, herbaceous plant species which has been widely known for its medicinal use. This study is aimed at the development of P. tenuifolia petal dry methanolic extract-loaded liposomes and the determination of their antioxidant activity. The liposomes were prepared with three different phos...

By Natalija Cutovic, Tatjana Marković, Ana Žugić, Petar Batinic, Branko Bugarski, Aleksandra Jovanović

This study aimed to assess the optimal extraction parameters, solvent-to-solid ratio, and extraction time, for ultrasound-assisted extraction of Satureja montana L. (winter savory) harvested from a 6-year-old experimental plantation. The optimization was performed in order to acquire extracts that are rich in the content of polyphenols (TPC), flavo...

By Natalija Čutović, Petar Batinić, Tatjana Marković, Dragoja Radanović, Aleksandar Marinković, Branko Bugarski, Aleksandra Jovanović

In the present study, basil (Ocimum basilicum L.) and lovage (Levisticum officinale Koch.) extracts were obtained using maceration, ultrasound- and microwave-assisted extractions (UAE and MAE, respectively). Varying the different extraction solvents, including water, methanol, acetone, and ethyl acetate, the analyses of total polyphenol and flavono...

By Petar Batinić, Natalija Čutović, Snežana Mrđan, Aleksandra Jovanović, Katarina Čirić, Aleksandar Marinković, Branko Bugarski

Even though Melissa officinalis L. (lemon balm, fam. Lamiaceae) is a well-known medicinal, aromatic, and spicy plant, and its physicochemical profile and biological activity have been investigated, there is no detailed research regarding the influence of solvent nature (polarity) on the extraction of active compounds (total polyphenols and total fl...

By Aleksandra Jovanović, Milica Mosurović, Branko Bugarski, Petar Batinić, Natalija Čutović, Stefan Gordanić, Tatjana Marković

The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio and e...

By Aleksandra Jovanović, Mihaela Skrt, Predrag Petrović, Gordana Zdunić, Katarina Šavikin, Branko Bugarski

Various conventional (maceration and heat-assisted extraction) and new alternative techniques (ultrasound-assisted extraction and microwave-assisted extraction) have been developed for the extraction of polyphenols compounds from different plant sources. Novel procedures have established with the aim to reduce the extraction time and solvent consum...

By Aleksandra Jovanović, Predrag Petrović, Verica Đorđević, Gordana Zdunić, Katarina Šavikin, Branko Bugarski

The Lamiaceae family, which contains about 200 genus and 3000 species, has a cosmopolitan distribution. Plants of this family are rich in essential oils and phenol compounds. Thymus vulgaris (Thyme) is perennial, subshrub plant which belongs to family of Labiates. Calyx and corolla are covered by dense gleaming glands that eradiate essential oil. L...

By Aleksandar Čančarević, Branko Bugarski, Katarina Šavikin, Gordana Zdunić