In the present study, basil (Ocimum basilicum L.) and lovage (Levisticum officinale Koch.) extracts were obtained using maceration, ultrasound- and microwave-assisted extractions (UAE and MAE, respectively). Varying the different extraction solvents, including water, methanol, acetone, and ethyl acetate, the analyses of total polyphenol and flavonoid contents (TPC and TFC, respectively), as well as antioxidant properties (DPPH radical scavenging and cupric ion reducing activities) were carried out for all obtained plants' extracts. The total amount of extractive substances of the selected extracts was also measured. The highest TPC was achieved in water basil extract obtained using UAE, while the highest TFC was determined in the basil extracts prepared using maceration and MAE. The highest level of DPPH radical neutralization was observed for methanol and acetone extracts obtained by maceration (for both plants) as well as UAE and MAE (for lovage). However, the obtained results did not exhibit statistically significant correlation with the TPC and TFC. The highest cupric ion-reducing capacity was measured in methanol lovage extract prepared using UAE and in methanol basil and lovage extracts from MAE. Interestingly, a significantly higher amount of the extractive substances was measured in all methanolic lovage extracts compared to basil parallels. Thus, it can be concluded that the selection of the extraction medium and extraction technique depends on the used plant species, as well as on the future application and purpose (or role) of the prepared extracts.
Amrani, S., Harnafi, H., Gadi, D., Mekhfi, H., Legssyer, A., Aziz, M., Martin-Nizard, F., & Bosca, L. (2009). Vasorelaxant and anti-platelet aggregation effects of aqueous Ocimum basilicum extract. Journal of Ethnopharmacology, 125(1), 157–162.
Anand, T., Sundararajan, M., Anbukkarasi, M., Thomas, P. A., & Geraldine, P. (2019). A methanolic extract of Ocimum basilicum exhibits antioxidant effects and prevents selenite-induced cataract formation in cultured lenses of wistar rats. Pharmacognosy Journal, 11(3), 496–504.
Anokwuru, C., Nduka, A., & Olusola, A. (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three Nigerian medicinal plants. Nature and Science of Sleep, 9(9), 53–61.
Bora, K. S., Arora, S., & Shri, R. (2011). Role of Ocimum basilicum L. in prevention of ischemia and reperfusion-induced cerebral damage, and motor dysfunctions in mice brain. Journal of Ethnopharmacology, 137(3), 1360–1365.
Chávez-González, M. L., Sepúlveda, L., Verma, D. K., Luna-García, H. A., Rodríguez-Durán, L. V., Ilina, A., & Aguilar, C. N. (2020). Conventional and emerging extraction processes of flavonoids. Processes, 8(4), 434–434.
Downey, M. O., & Hanlin, R. L. (2016). Comparison of ethanol and acetone mixtures for extraction of condensed tannin from grape skin. South African Journal of Enology and Viticulture, 31(2).
Ethyl lactate as a solvent: Properties, applications and production processes: A review. (2011). Green Chemistry, 13(10), 2658–2658.
Ghanta, S., Bhaumik, C., & Manna, M. S. (2022). Process development for isolation of dietary eugenol from leaves of basil (Ocimum sanctum) in combination of optimization of process variables and modeling by artificial neural network. Journal of the Indian Chemical Society, 99(1), 100280–100280.
Gil-Chávez, J. G., Villa, J. A., Fernando, A. J., Basilio, H. J., Sepulveda, D., Yahia, E. M., & González-Aguilar, G. A. (2013). Technologies for extraction and production of bioactive compounds to be used as nutraceuticals and food ingredients: An overview. Comprehensive Reviews in Food Science and Food Safety, 12(1), 5–23.
Gülçin, İ., Elmastaş, M., & Aboul-Enein, H. Y. (2007). Determination of antioxidant and radical scavenging activity of Basil (Ocimum basilicum L. Family Lamiaceae) assayed by different methodologies. Phytotherapy Research, 21(4), 354–361.
Hirano, R., Sasamoto, W., Matsumoto, A., Itakura, H., Igarashi, O., & Kondo, K. (2001). Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation. Journal of Nutritional Science and Vitaminology, 47(5), 357–362.
Ilić, A. S., Antić, M. P., Jelačić, S. C., & Šolević, K. T. M. (2018). Chemical composition of the essential oils of three Ocimum basilicum L. cultivars from Serbia. Notulae Botanicae Horti Agrobotanici ClujNapoca, 47(2), 347–351.
Jacotet-Navarro, M., Rombaut, N., Fabiano-Tixier, A.-S., Danguien, M., Bily, A., & Chemat, F. (2015). Ultrasound versus microwave as green processes for extraction of rosmarinic, carnosic and ursolic acids from rosemary. Ultrasonics Sonochemistry, 27, 102–109.
Jayasinghe, C., Gotoh, N., Aoki, T., & Wada, S. (2003). Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L. Journal of Agricultural and Food Chemistry, 51(15), 4442–4449.
Jones, W. P., Kinghorn, D. A., S.D., S., Z., L., & A, G. (2006). Extraction of plant secondary metabolites (pp. 323–351).
Jovanović, A. A., Djordjević, V. B., Petrović, P. M., Pljevljakušić, D. S., Zdunić, G. M., Šavikin, K. P., & Bugarski, B. M. (2021). The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme. Journal of Applied Research on Medicinal and Aromatic Plants, 25, 100328–100328.
Jovanović, A. A., Ðorđević, V. B., Zdunić, G. M., Pljevljakušić, D. S., Šavikin, K. P., Gođevac, D. M., & Bugarski, B. M. (2017). Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat-and ultrasound-assisted techniques. Separation and Purification Technology, 179, 369–380.
Jovanović, A. A., Petrović, P. M., Đorđević, V. B., Zdunić, G. M., Savikin, K. P., & Bugarski, B. M. (2021). Free radicals’ scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques. Hrana i Ishrana, 62, br. 2, 15–20.
Jovanović, A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K., & Bugarski, B. (2017). Polyphenols extraction from plant sources. Lekovite Sirovine, Br, 37, 45–49.
Jovin, E., Toth, A., Beara, I., Balog, K., Orčić, D., & Mimica-Dukić, N. (2008). Chemical composition and antioxidant capacity of Marrubium peregrinum L. from Vojvodina (Serbia. Planta Medica, 74(09).
Khan, I. A., & Abourashed, E. A. (2010). Leung’s encyclopedia of common natural ingredients used in food, drugs, and cosmetics.
Kozłowska, M., Ścibisz, I., Przybył, J., Ziarno, M., Żbikowska, A., & Majewska, E. (2021). Phenolic contents and antioxidant activity of extracts of selected fresh and dried herbal materials. Polish Journal of Food and Nutrition Sciences, 269–278.
Leal, P. F., Maia, N. B., Carmello, Q. A. C., Catharino, R. R., Eberlin, M. N., & Meireles, M. A. A. (2008). Sweet basil (Ocimum basilicum) extracts obtained by supercritical fluid extraction (sfe): Global yields, chemical composition, antioxidant activity, and estimation of the cost of manufacturing. Food and Bioprocess Technology, 1(4), 326–338.
Liazid, A., Palma, M., Brigui, J., & Barroso, C. G. (2007). Investigation on phenolic compounds stability during microwave-assisted extraction. Journal of Chromatography A, 1140(1–2), 29–34.
Onyebuchi, C., & Kavaz, D. (2020). Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts. Scientific Reports, 10(1), 21760–21760.
Özyürek, M., Güçlü, K., Tütem, E., Başkan, K. S., Erçağ, E., Esin, Ç. S., Baki, S., Yıldız, L., Karaman, Ş., & Apak, R. (2011). A comprehensive review of CUPRAC methodology. Analytical Methods, 3(11), 2439–2439.
Pereira, R. P., Fachinetto, R., Souza, P. A., Puntel, R. L., Santos, D. S. G. N., Heinzmann, B. M., Boschetti, T. K., Athayde, M. L., Bürger, M. E., & Morel, A. F. (2009). Antioxidant effects of different extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus. Neurochemical Research, 34(5), 973–983.
Pérez-Jiménez, J., Arranz, S., Tabernero, M., Díaz-Rubio, E. M., Serrano, J., Goñi, I., & Saura-Calixto, F. (2008). Updated methodology to determine antioxidant capacity in plant foods, oils and beverages: Extraction, measurement and expression of results. Food Research International, 41(3), 274–285.
Poorhashemi, S., Arianfar, A., & Mohammadi, A. (2019). Ultrasoundassisted extraction and optimization process parameters of antioxidant and phenolic compounds from Myristica fragrans. Jundishapur Journal of Natural Pharmaceutical Products, 15(1).
Rajakaruna, A., Manful, C. F., Abu-Reidah, I. M., Critch, A. L., Vidal, N. P., Pham, T. H., Cheema, M., & Thomas, R. (2022). Application of solvent pH under pressurized conditions using accelerated solvent extraction and green solvents to extract phytonutrients from wild berries. Food Bioscience, 47, 101471–101471.
Ravindran, P. N., Pillai, G. S., & Nirmal, B. K. (2004). Under-utilized herbs and spices. u: Peter K.V (pp. 53–103).
Sharma, A., Pant, S., Wani, Z., & Mahajan, R. (2021). Understanding the barriers of underutilization and applicability of biotechnological tools for augmenting spice crop production: Neglected and underutilized cropstowards nutritional security and sustainability (pp. 237–266).
Snoussi, M., Dehmani, A., Noumi, E., Flamini, G., & Papetti, A. (2016). Chemical composition and antibiofilm activity of Petroselinum crispum and Ocimum basilicum essential oils against Vibrio spp. strains. Microbial Pathogenesis, 90, 13–21.
Stanković, M. S. (2011). Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac Journal of Science, 33(1), 63–72.
Teofilović, B. (2016). Biochemical and chemical characterization of basil extracts and influence of pharmaceutical technological formulations on the glycemic, lipid and oxidative status in experimental animals.
Thorough study of reactivity of various compound classes toward the Folin-Ciocalteu reagent. (2010). Journal of Agricultural and Food Chemistry, 58(14), 8139–8144.
Tumbas, Š. V., Kovačević, S., Šeregelj, V., Šovljanski, O., Mandić, A., Ćetković, G., Vulić, J., Podunavac-Kuzmanović, S., & Čanadanović-Brunet, J. (2021). Improvement of carrot accelerated solvent extraction efficacy using experimental design and chemometric techniques. Processes, 9(9), 1652–1652.
Veronezi, C. M., Costa, T., & Jorge, N. (2014). Basil (Ocimum basilicum L.) as a natural antioxidant. Journal of Food Processing and Preservation, 38(1), 255–261.
Warsi, S., & A.R. (2017). Phytochemical screening and antioxidant activity of ethanolic extract and ethyl acetate fraction from basil leaf (Ocimum basilicum L.) by DPPH radical scavenging method. IOP Conference Series: Materials Science and Engineering, 259, 012008–012008.
Zengin, G., Ferrante, C., Orlando, G., Zheleva-Dimitrova, D., Gevrenova, R., Recinella, L., Chiavaroli, A., Leone, S., Brunetti, L., Aumeeruddy, M. Z., Aktumsek, A., & Mahomoodally, M. F. (2019). Chemical profiling and pharmaco-toxicological activity of Origanum sipyleumextracts: Exploring for novel sources for potential therapeutic agents. Journal of Food Biochemistry, 43(11), 1–16.
Zuhair, R., Aminah, A., & Sahilah, A. (2013). Effect of extraction solvents on the phenolic content and antioxidant properties of two papaya cultivars. Journal of Medicinal Plants Research, 7(46), 3354–3359.
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Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Institut za proučavanje lekovitog bilja 'Dr Josif Pančić', Beograd) (MPNTR - 451-03-68/2020-14/200003)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (MPNTR - 451-03-68/2020-14/200287)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (MPNTR - 451-03-68/2020-14/200135)
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Univerzitet u Beogradu, Institut za primenu nuklearne energije - INEP) (MPNTR - 451-03-68/2020-14/200019)
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