×
Home Current Archive Editorial board
News Contact
Research Article

Morphological and anatomical analysis of the clary sage herbal drug (Salviae sclareae herba)

By
Milica Ranđelović ,
Milica Ranđelović
Contact Milica Ranđelović

Department of Pharmacy, Faculty of Medicine, University of Nis , Niš , Serbia

Bojan Zlatković ,
Bojan Zlatković

Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Nis , Niš , Serbia

Maja Jovanović ,
Maja Jovanović

Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Nis , Niš , Serbia

Bojana Miladinović ,
Bojana Miladinović

Department of Pharmacy, Faculty of Medicine, University of Nis , Niš , Serbia

Milica Milutinović ,
Milica Milutinović

Department of Pharmacy, Faculty of Medicine, University of Nis , Niš , Serbia

Dragana Pavlović ,
Dragana Pavlović

Department of Pharmacy, Faculty of Medicine, University of Nis , Niš , Serbia

Suzana Branković ,
Suzana Branković

Department of Physiology, Faculty of Medicine, University of Nis , Niš , Serbia

Dušanka Kitić Orcid logo
Dušanka Kitić

Department of Pharmacy, Faculty of Medicine, University of Nis , Niš , Serbia

Abstract

This paper presents an analysis of the morphological and anatomical characteristics of the aboveground parts (herba) of clary sage (Salvia sclarea L.), with an emphasis on the investigation of its surface structures. After the procedure of the primary processing and drying of the plant material, it was determined that the composition of the crushed herbal drug Salviae sclareae herba showed the presence of parts of leaves, bracts, flowers and axis of the inflorescence, stems, and a small amount of ripe nuts. The analysis of the surface structures of the drug parts on a stereomicroscope and then on permanent microscopic slides indicated the presence of two functional types of trichomes (glandular and non-glandular). Non-glandular mechanical trichomes are found in the unicellular and multicellular forms while glandular trichomes are differentiated as capitate, with a greater number of subtypes, and peltate. Peltate trichomes are most common on the calyx and corolla while capitate are most abundant on the surface of the stem and leaf. Further studies should be directed toward the chemical characterization of the essential oil of the herb and individual organs present in the glandular trichomes, along with the investigation of its pharmacological effects.

References

Aćimović, M., Todosijević, M., Varga, A., Tešević, V., Čabarkapa, I., Kiprovski, B., & Sikora, V. (2019). Bioactivity of essential oils from cultivated winter savory, sage and hyssop. Lekovite Sirovine, 39, 11–17.
Alimpić, A., Lam., S. jurisicii K., Sibth., S., & Sm. (2016). Macromorphological characteristics of Salvia amplexicaulis.
Aradski-Alimpić, A., Oalđe-Pavlović, M., Janković, S., Marin, P. D., & Duletić-Laušević, S. (2021). Enzyme inhibitors as controllers of neurodegenerative diseases: An update of in vitro effects of medicinal plants. Lekovite Sirovine, 41, 72–105.
Aromatic plants of Serbia. (1995).
Bini, M. L. (1988). Structure and mode of secretion of glandular trichomes in clary sage (Salvia sclarea L. Mem. Atti Della Societa Toscana Di Scienze Naturali, Memorie Seri B, 95, 183–197.
Chakalova, E., Genova, E., & Thuy, N. (1993). Comparative anatomical investigations on leaves of Salvia officinalis L. And S. Tomentosa Mill. Fitologija, 46, 3–11.
Díaz, D. P., Cabrera, A., Alem, D., Larrañaga, P., Ferreira, F., & Dalla, R. M. (2011). Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chilean Journal of Agricultural Research, 71(2), 231–239.
Diklić, N., u, G. S. L., & M, J. (1974). Flora of Republic of Serbia.
Dweck, A. (2000). Folklore and cosmetic use of various Salvia species. u: Kintzios S. In ur.] Sage: The Genus Salvia.
Eiji, S., & Salmaki, Y. (2016). Evolution of trichomes and its systematic significance in Salvia (Mentheae; Nepetoideae; Lamiaceae. Botanical Journal of the Linnean Society, 180(2), 241–257.
Guarrera, P. M. (2005). Traditional phytotherapy in Central Italy. Fitoterapia, 76(1), 1–25.
Hagers handbuch der pharmazautische praxis: Drogen P-Z. (1993).
Imanshahidi, M., & Hosseinzadeh, H. (2006). The pharmacological effects of Salvia species on the central nervous system. Phytotherapy Research, 20(6), 427–437.
Jančić, R. (2002). Pharmaceutical botany. In glasnik, in Serbian.
Koutsaviti, A., Tzini, D. I., & Tzakou, O. (2016). Greek Salvia sclarea L. essential oils: Effect of hydrodistillation time, comparison of the aroma chemicals using hydrodistillation and HS-SPME techniques. Rec. Nat. Prod, 6–6.
Krstic, L., Malencic, D., & Anackov, G. (2006). Structural investigations of trichomes and essential oil composition of Salvia verticillata. Botanica Helvetica, 116(2), 159–168.
Leporatti, M. L., Pavesi, A., & Posocco, E. (1985). Phytotherapy in the Valnerina Marche (Central Italy. Journal of Ethnopharmacology, 14(1), 53–63.
Metcalfe, C. R., & Chalk, L. (1980). Anatomy of the dicotyledons: Volume I: Systematic anatomy of leaf and stem, with a brief history of the subject.
Özdemir, C., & Senel, G. (1999). The morphological, anatomical and karyological properties of Salvia sclarea L. Turkish Journal of Botany, 23, 7–18.
Peana, A. T., & Moretti, M. D. (2002). Pharmacological activities and applications of Salvia sclarea and Salvia desoleana essential oils. Studies in Natural Products Chemistry, 26, 391-423,.
Sarić, M. (1989). Madicinal plants of Serbia.
Schmiderer, C., Grassi, P., Novak, J., Weber, M., & Franz, C. (2008). Diversity of essential oil glands of clary sage (Salvia sclarea L., Lamiaceae. Plant Biology, 10(4), 433–440.
Stojičić, D., Tošić, S., Slavkovska, V., Zlatković, B., Budimir, S., Janošević, D., & Uzelac, B. (2016). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. Lamiaceae). Planta, 244(2), 393–404.
Walker, J. B., Sytsma, K. J., Treutlein, J., & Wink, M. (2004). Salvia (Lamiaceae) is not monophyletic: Implications for the systematics, radiation, and ecological specializations of Salvia and tribe Mentheae. American Journal of Botany, 91(7), 1115–1125.
Zlatković, B., Ranđelović, V., Jušković, M., & Marković, M. (2005). New floristic records in Serbia and Northern Macedonia. U: 8th Symposium on Flora of Southeastern Serbia and Neighbouring Regions, Niš: Faculty of Sciences and Mathematics - Department of Biology and Ecology, 1–4.
(2000).
(2020). European pharmacopoeia. Strasbourg: Council of Europe -Directorate for the quality of medicines and healthcare. Ph.Eur, 10.

Citation

Funding Statement

Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Institut za proučavanje lekovitog bilja 'Dr Josif Pančić', Beograd) (MPNTR - 451-03-68/2020-14/200003) Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Univerzitet u Nišu, Medicinski fakultet) (MPNTR - 451-03-68/2020-14/200113)

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

Article metrics

Google scholar: See link

The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.