This paper studies the effect of gibberellic acid (GA3), herbal preparation (LAB1) and the pre-cooling treatment (Ph) on seed germination of Origanum heracleoticum L. and Origanum vulgare L. As a control variant, seed treatment with distilled water was used. The highest germination energy (EK), germination after 14 days (14D) and total germination values (UK) were achieved with seeds of O. heracleoticum treated with 400 ppm GA3 (50.3%, 57.3% and 64.8%, respectively) and the lowest values were observed with 3-day pre-cooling treatment (28.3%, 28.8% and 28.8%, respectively). The highest values for all seed quality parameters for O. vulgare were achieved by the treatment with the highest GA3 concentration, 1000 ppm (60.0%, 67.0% and 74.8%, respectively), while the lowest germination values were recorded in 4-day pre-cooling treatment (26.0%, 26.8% and 27.5%, respectively). Overall, higher germination was observed in seeds of O. vulgare. The best germination stimulitor for both species proved to be gibberellic acid (GA3), a hormone of plant origin, followed by the herbal preparation (LAB1), then the control variant (DW) and, as the most unfavourable to stimulate germination proved to be pre-cooling treatment (Ph), which had no impact on the increase of seed germination.
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