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Originalan naučni rad– Original scientific paper

CHEMICAL COMPOSITION AND INHIBITORY ACTIVITY OF SELECTED ESSENTIAL OILS AGAINST FUNGI ISOLATED FROM MEDICINAL PLANTS

By
Tatjana Stević ,
Tatjana Stević

Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia

Slaviša Stanković ,
Slaviša Stanković

Faculty of Biology, University of Belgrade, Belgrade, Serbia

Katarina Šavikin ,
Katarina Šavikin

Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia

Dejan Gođevac ,
Dejan Gođevac

Institute for Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia

Ivica Dimkić ,
Ivica Dimkić

Faculty of Biology, University of Belgrade, Belgrade, Serbia

Marina Soković
Marina Soković

Institute for Biological Research “Siniša Stanković”, Belgrade, Serbia

Abstract

In recent years great attention was paid to biological control, the application of natural products in order to protect crops and medicinal plants against contamination with phytopathogenic and saprophytic fungi. Essential oils have been evaluated as a potentially safe replacement for chemicals used for that purpose. In this regard, antifungal potential of six essential oils was examined coupled with determination of the complete qualitative and quantitative chemical composition by GC-FID and GC-MS analysis. While essential oils of rosemary, sage, Spanish sage and black pepper were exclusively composed of monoterpenes, vetiver essential oil was entirely composed of sesquiterpenes. In essential oil of cinnamon dominates (E)-cinnamaldehyde. Applying the in vitro microdilution method, it was found that all essential oils were active in inhibiting the growth of all tested 21 pre- and post-harvest phytopathogenic and saprophytic fungi. MIC and MFC ranged from 1.2 mg ml-1 up to 22.6 mg ml-1 according to the test oil. Rosemary oil showed the best antifungal potential, followed by black pepper and cinnamon oil. Sage and Spanish sage oils also exhibited significant antifungal potential. Vetiver oil demonstrated the lowest antifungal activity. Essential oils that showed considerable antifungal potential are good candidates for further examination of their use in preventing and/or protection of medicinal plants, their seeds and dried drugs against fungal infections, both in the field and in warehouses.


 

References

Abdel-Kader, M., El-Mougy, N., & Lashin, S. (2011). Essential oils and Trichoderma harzianum as an integrated control measure against faba bean root pathogens. J. Plant Prot. Res, 51(3), 306 313.
Bouzenna, H., & Krichen, L. (2013). Pelargonium graveolens L’Her. andArtemisia arborescens L. essential oils: Chemical composition, antifungal activity against Rhizoctonia solani and insecticidal activity against Rhysopertha dominica. Nat. Prod. Res, 27(9, 841-846 78.
Champagnat, P., Figueredo, G., Chalchat, J. C., Carnat, A. P., & Bessibre, J. M. (2006). A study on the composition of commercial Vetiveria zizanioldes oils from different geographical origins. J. Essent. Oil Res.,Vol, 18, 416–422.
Daouk, R., Dagher, H., & Sattout, J. (1995). Antifungal activity of the essential oil of Origanum syriacum L. J. Food Protect.,Vol, 58, 1147–1149.
Deba, F., Xuan, T. D., Yasuda, M., & Tawata, S. (2008). Chemical composition and antioxidant, antibacterial and antifungal activities of the essential oils from Bidens pilosa Linn. Var. Radiate, Food Control,Vol, 19, 346–352.
Hanel, H., & Raether, W. (1988). A more sophisticated method of determining the fungicidal effect of water-insoluble preparations with a cell harvester, using miconazole as an example. Mycoses,Vol, 31, 148–154.
Isman, B. M. (2000). Plant essential oils for pest and disease management. Crop Prot, 19, 603–608.
Jiang, Y., Wu, N., Fu, Y.-J., Wang, W., Luo, M., & Zhao, C. J. (2011). Chemical composition and antimicrobial activity of the essential oil of Rosemary, Environ. Toxicol. Pharmacol, 32, 63–68.
Kalemba, D., & Kunicka, A. (2003). Antibacterial and antifungal properties of essential oils, Curr. Med. Chem, 10, 813–829.
Krauze-Baranowska, M., Mardarowicz, M., Wiwart, M., Poblocka, L., & Dynowska, M. (2002). Antifungal activity of the essential oils from some species of the genus Pinus. Verlag Der Zeitschrift Fur Naturforschung, 57c, 478–482.
Lakušić, D., Ristić, M., Slakovska, V., & Lakušić. (2013). Seasonal variations in the composition of the essential oils of rosemary (Rosmarinus officinalis, Lamiaceae. Nat. Prod. Commun, 8, 131–134.
Lee, S. Y., Kim, S. H., Hong, C. Y., Park, M.-J., & I.-GChoi. (2013). Effects of (−)-borneol on the growth and morphology of Aspergillus fumigatus and Epidermophyton floccosom. Flavor Frag, J.,vol.28(2, 129–134.
Lopez-Reyes, J. G., Spadaro, D., Prelle, A., Garibaldi, A., & Gullino, M. L. (2013). Efficacy of plant essential oils on postharvest control of rots by fungi on different stone fruits in vivo. J. Food Prot, 76(4), 631–639.
Magiatis, P., Melliou, E., Skaltsounis, A. L., Chinou, I. B., & Mitaku, S. (1999). Chemical composition and antimicrobial activity of the essential oils of Pistacia lentiscus var. chia. Planta Medica,Vol, 65, 749–752.
Menon, A. N., Padmakumari, K. P., Jayalekshmy, A., Gopalakrishnan, M., & Narayanan, C. S. (2000). Essential Oil Composition of Four Popular Indian Cultivars of Black Pepper (Piper nigrum L. J. Essent. Oil Res.,Vol, 12, 431–434.
Mockuté, D., D., O. N., Bernotiené, G., & Butkiené, R. (2003). The cisthujone chemotype of Salvia officinalis L. Essential Oils, Chemija (Vilnius),Vol, 14(4), 216–220.
Ooi, L. S. M., Li, Y. L., Kam, S. L., Wang, H., Wong, E. Y. L., & Ooi, V. E. C. (2006). Antimicrobial activities of cinnamon oil and cinnamaldehyde from the Chinese medicinal herb Cinnamomum cassia. Blume, Am. J. Chin. Med.,Vol, 34, 511–522.
Perry, N. S. L., Houghton, P. J., Theobald, A., Jenner, P., & Perry, E. K. (2000). Invitro inhibition of human erythrocyte acetylcholinesterase by Salvia lavandulaefolia essential oil and constituent terpenes. J. Pharm. Pharmacol, 52, 895–902.
Pitarokili, D., Tzakou, O., Couladis, M., & Verykokidou, E. (1999). Composition and antifungal activity of the essential oil of Salvia pomifera subsp. calycina.
Rapp, R. P. (2004). Changing strategies for the management of invasive fungal infections. Pharmacotherapy, 24, 4–28.
Singh, G., Maurya, S., DeLampasona, M. P., & Catalan, C. A. N. (2007). A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food Chem. Toxicol, 45, 1650–1661.
Skandamis, P., Koutsoumanis, K., Fasseas, K., & Nychas, G. J. E. (2001). Inhibition of oregano essential oil and EDTA on E. Coli O157:H7,Ital. J. Food Sci, 13, 55–65.
Stević, T., Pavlović, S., Stanković, S., & Šavikin, K. (2012). Pathogenic microorganisms of medicinal herbal drugs, Arch. Biol. Sci, 64(1), 49–58.
Tabassum, N., & G.M, V. (2013). Antifungal investigations on plant essential oils. A review. Int. J. Pharm. Pharm. Sci.,Vol, 5(2), 19–28.
Tomaino, F. C., Zimbalati, V., Venuti Enuti, V., Sulfaro, V., & Pasquale, A. (2005). Influence of heating on antioxidant activity and the chemical composition of some spice essential oils”. Food Chem.,Vol, 89, 549–554.
Vilela, G. R., De Almeida, G. S., D’Arce, M. A., Moraes, M. H. D., Brito, J. O., & Da Silva, M. F. (2009). Activity of essential oil and its major compound, 1,8-cineole, from Eucalyptus globulus Labill., against the storage fungi. Aspergillus Flavus Link and Aspergillus Parasiticus Speare, J. Stored Prod. Res, 45, 108–111.
Zuzarte, M., M., M. J. G., Cavaleiro, C., Dinis, A. M., Canhoto, J., & Salgueiro, L. (2009). Chemical composition and antifungal activity of the essential oils of Lavandulapedunculata (Miller. Cav. Chem. Biodivers, 6, 1283–1292.

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