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Originalan naučni rad– Original scientific paper

IN VITRO PRODUCTION OF CHLOROGENIC ACID IN CULTURE OF TRANSFORMED CICHORIUM INTYBUS L. PLANTS

By
Milica Bogdanović ,
Milica Bogdanović

Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

Milan Dragićević ,
Milan Dragićević

Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

Angelina Subotić ,
Angelina Subotić

Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

Ana Simonović ,
Ana Simonović

Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

Slađana Todorović
Slađana Todorović

Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

Abstract

Chicory (Cichorium intybus L.) is traditionally recognized for its anti-inflammatory, antimicrobial, anticancer and nutritive properties. Among active secondary metabolites detected in chicory, the most important are sesquiterpene lactones and phenolics, including chlorogenic acid (CA). Hereby we have analyzed the content of CA in previously obtained Agrobacterium rhizogenes – transformed chicory hairy root cultures and transformed regenerants. Among three analyzed hairy root clones, clone 13 had exceptionally high biomass production, so the amount of CA in this culture was the highest, e.g. 400 times higher in comparison to the untransformed root culture. Since the roots spontaneously regenerated, the system was upgraded to allow the comparison of CA content not only among the clones, but also between different developmental phases of the regenerants (vegetative vs. flowering plants) and their organs (roots vs. leaves). It was shown that the CA production varies from clone to clone, and also depends on the phenophase of the clone. In the rosette stage of clones 13 and 36, the highest amount of CA was detected in roots, while the same clones in the flowering stage had significantly lower CA content. The clone 35 in the vegetative phase produced the lowest amounts of CA. However, in the flowering stage the roots of clone 35 produced the highest CA amount. Among the regenerants, the clone 13 had the fastest growth, and hence the best CA production.

The current paper presents for the first time the CA content in transformed chicory regenerants. The obtained results suggest that the culture of transformed chicory plants can be equally good source of CA as liquid hairy root culture.


 

References

[{"id":750,"citationNumber":"1.","type":"article-journal","title":"Cichorium intybus: Traditional Uses, Phytochemistry, Pharmacology, and Toxicology","DOI":null,"author":[{"given":"R.A.","family":"Street"},{"given":"J.","family":"Sidana"},{"given":"G.","family":"Prinsloo"}],"issued":{"date-parts":["2013"]},"container-title":"Evidence-Based Complementary and Alternative Medicine","volume":"2013","issue":null,"page":"1\u2013 13 64"}]

[{"id":751,"citationNumber":"2.","type":"article-journal","title":"Cultivation and breeding of root chicory for inulin production","DOI":null,"author":[{"given":"J.R.A.","family":"Baert"},{"given":"E.J.","family":"Bockstaele"}],"issued":{"date-parts":["1992"]},"container-title":"Industrial Crops and Products","volume":"1","issue":"2\u20134","page":"229\u2013 234"}]

[{"id":752,"citationNumber":"3.","type":"article-journal","title":"Phytochemical and antibacterial studies of Chicory (Cichorium intybus L.)-A multipurpose medicinal plant","DOI":null,"author":[{"given":"S.","family":"Nandagopal"},{"given":"B.R.","family":"Kumari"}],"issued":{"date-parts":["2007"]},"container-title":"Advances in Biological Research","volume":"1","issue":"1-2","page":"17\u201321"}]

[{"id":753,"citationNumber":"4.","type":"article-journal","title":"Guaianolides from Cichorium intybus and structure revision of Cichorium sesquiterpene lactones","DOI":null,"author":[{"given":"W.","family":"Kisiel"},{"given":"K.","family":"Zieli\u0144ska"}],"issued":{"date-parts":["2001"]},"container-title":"Phytochemistry","volume":"57","issue":"4","page":"523\u2013527"}]

[{"id":754,"citationNumber":"5.","type":"article-journal","title":"Production of uaianolides in Agrobacterium rhizogenes transformed chicory regenerants flowering in vitro","DOI":null,"author":[{"given":"M.D.","family":"Bogdanovi\u0107"},{"given":"S.I.","family":"Todorovi\u0107"},{"given":"T.","family":"Banjanac"},{"given":"M.B.","family":"Dragi\u0107evi\u0107"},{"given":"F.W.A.","family":"Verstappen"},{"given":"H.J.","family":"Bouwmeester"},{"given":"A.D.","family":"Simonovi\u0107"}],"issued":{"date-parts":["2014"]},"container-title":"Industrial Crops and Products","volume":"60","issue":null,"page":"52\u201359"}]

[{"id":755,"citationNumber":"6.","type":"article-journal","title":"Polyphenol content and antiradical activity of Cichorium intybus L. from biodynamic and conventional farming","DOI":null,"author":[{"given":"D.","family":"Heimler"},{"given":"L.","family":"Isolani"},{"given":"P.","family":"Vignolini"},{"given":"A.","family":"Romani"}],"issued":{"date-parts":["2009"]},"container-title":"Food Chemistry","volume":"114","issue":"3","page":"765\u2013770"}]

[{"id":756,"citationNumber":"7.","type":"article-journal","title":"Antioxidant Activities of Caffeic Acid and Its Related Hydroxycinnamic Acid Compounds","DOI":null,"author":[{"given":"J.H.","family":"Chen"},{"given":"C.-T.","family":"Ho"}],"issued":{"date-parts":["1997"]},"container-title":"Journal of Agricultural and Food Chemistry","volume":"45","issue":"7","page":"2374\u20132378"}]

[{"id":757,"citationNumber":"8.","type":"article-journal","title":"Inhibition of Activator Protein-1, NF-\u03baB, and MAPKs and Induction of Phase 2 Detoxifying Enzyme Activity by Chlorogenic Acid","DOI":null,"author":[{"given":"R.","family":"Feng"},{"given":"Y.L.L.","family":"Lu"},{"given":"Y.","family":"Bowman"},{"given":"V.","family":"Qian"},{"given":"M.","family":"Castranova"},{"given":"M.","family":"Ding"}],"issued":{"date-parts":["2005"]},"container-title":"Journal of Biological Chemistry","volume":"280","issue":"30","page":"27888\u201327895"}]

[{"id":758,"citationNumber":"9.","type":"article-journal","title":"Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids","DOI":null,"author":[{"given":"B.","family":"\u00d6z\u00e7elik"},{"given":"M.","family":"Kartal"},{"given":"I.","family":"Orhan"}],"issued":{"date-parts":["2011"]},"container-title":"Pharmaceutical biology","volume":"49","issue":"4","page":"396\u2013402"}]

[{"id":759,"citationNumber":"10.","type":"article-journal","title":"Anti-inflammatory activity of Cymbopogon citratus leaves infusion via proteasome and nuclear factor-\u03baB pathway inhibition: Contribution of chlorogenic acid","DOI":null,"author":[{"given":"V.","family":"Francisco"},{"given":"G.","family":"Costa"},{"given":"A.","family":"Figueirinha"},{"given":"C.","family":"Marques"},{"given":"P.","family":"Pereira"},{"given":"B.Miguel","family":"Neves"},{"given":"M.Celeste","family":"Lopes"},{"given":"C.","family":"Garc\u00eda-Rodr\u00edguez"},{"given":"M.Teresa","family":"Cruz"},{"given":"M.Teresa","family":"Batista"}],"issued":{"date-parts":["2013"]},"container-title":"Journal of Ethnopharmacology","volume":"148","issue":"1","page":"126\u2013134"}]

[{"id":760,"citationNumber":"11.","type":"article-journal","title":"Roles of Chlorogenic Acid on Regulating Glucose and Lipids Metabolism","DOI":null,"author":[{"given":"S.","family":"Meng"},{"given":"J.","family":"Cao"},{"given":"Q.","family":"Feng"},{"given":"J.","family":"Peng"},{"given":"Y.","family":"Hu"}],"issued":{"date-parts":["2013"]},"container-title":"A Review, Evidence-Based Complementary and Alternative Medicine","volume":"2013","issue":null,"page":"1\u201311"}]

[{"id":761,"citationNumber":"12.","type":"article-journal","title":"Radioprotection by two phenolic compounds: Chlorogenic and quinic acid, on X-ray induced DNA damage in human blood lymphocytes in vitro","DOI":null,"author":[{"given":"N.","family":"Cinkilic"},{"given":"S.K.","family":"Cetintas"},{"given":"T.","family":"Zorlu"},{"given":"O.","family":"Vatan"},{"given":"D.","family":"Yilmaz"},{"given":"T.","family":"Cavas"},{"given":"S.","family":"Tunc"},{"given":"L.","family":"Ozkan"},{"given":"R.","family":"Bilaloglu"}],"issued":{"date-parts":["2013"]},"container-title":"Food and Chemical Toxicology","volume":"53","issue":null,"page":"359\u2013363 65"}]

[{"id":762,"citationNumber":"13.","type":"article-journal","title":"Plant cell cultures: Chemical factories of secondary metabolites","DOI":null,"author":[{"given":"S.Ramachandra","family":"Rao"},{"given":"G.A.","family":"Ravishankar"}],"issued":{"date-parts":["2002"]},"container-title":"Biotechnology Advances","volume":"20","issue":"2","page":"101\u2013 153"}]

[{"id":763,"citationNumber":"14.","type":"article-journal","title":"Production of plant secondary metabolites: a historical perspective","DOI":null,"author":[{"given":"F.","family":"Bourgaud"},{"given":"A.","family":"Gravot"},{"given":"S.","family":"Milesi"},{"given":"E.","family":"Gontier"}],"issued":{"date-parts":["2001"]},"container-title":"Plant Science","volume":"161","issue":"5","page":"839\u2013851"}]

[{"id":764,"citationNumber":"15.","type":"article-journal","title":"Putrescine Influences Growth and Production of Coumarins in Hairy Root Cultures of Witloof Chicory (Cichorium intybus L. cv","DOI":null,"author":[{"given":"H.P.","family":"Bais"},{"given":"G.","family":"Sudha"},{"given":"G.A.","family":"Ravishankar"}],"issued":{"date-parts":["1999"]},"container-title":"Lucknow Local), Journal of Plant Growth Regulation","volume":"18","issue":"4","page":"159\u2013165"}]

[{"id":765,"citationNumber":"16.","type":"article-journal","title":"Long-Term Cultured Hairy Roots of Chicory \u2013 A Rich Source of Hydroxycinnamates and 8-Deoxylactucin Glucoside","DOI":null,"author":[{"given":"J.","family":"Malarz"},{"given":"A.","family":"Stojakowska"},{"given":"W.","family":"Kisiel"}],"issued":{"date-parts":["2013"]},"container-title":"Applied Biochemistry and Biotechnology","volume":null,"issue":null,"page":"1\u201313"}]

[{"id":766,"citationNumber":"17.","type":"article-journal","title":"Production of volatile compounds by hairy root cultures of Cichorium intybus L under the influence of fungal elicitors and their analysis using solid phase micro extraction gas chromatography\u2013mass spectrometry","DOI":null,"author":[{"given":"H.P.","family":"Bais"},{"given":"B.","family":"Dattatreya"},{"given":"G.","family":"Ravishankar"}],"issued":{"date-parts":["2003"]},"container-title":"Journal of the Science of Food and Agriculture","volume":"83","issue":"8","page":"769\u2013774"}]

[{"id":767,"citationNumber":"18.","type":"article-journal","title":"Modification of phenotype in Belgian endive (Cichorium intybus) through genetic transformation by Agrobacterium rhizogenes: conversion from biennial to annual flowering","DOI":null,"author":[{"given":"L.-Y.","family":"Sun"},{"given":"G.","family":"Touraud"},{"given":"C.","family":"Charbonnier"},{"given":"D.","family":"Tepfer"}],"issued":{"date-parts":["1991"]},"container-title":"Transgenic Research","volume":"1","issue":"1","page":"14\u201322"}]

[{"id":768,"citationNumber":"19.","type":"article-journal","title":"Direct Regeneration of Shoots from Hairy Root Cultures of Centaurium erythraea Inoculated with Agrobacterium rhizogenes","DOI":null,"author":[{"given":"A.","family":"Suboti\u0107"},{"given":"S.","family":"Budimir"},{"given":"D.","family":"Grubi\u0161i\u0107"},{"given":"I.","family":"Mom\u010dilovi\u0107"}],"issued":{"date-parts":["2003"]},"container-title":"Biologia Plantarum","volume":"47","issue":"4","page":"617\u2013619"}]

[{"id":769,"citationNumber":"20.","type":"article-journal","title":"Shoot and root culture of Hypericum perforatum L. transformed with Agrobacterium rhizogenes A4M70GUS","DOI":null,"author":[{"given":"B.","family":"Vinterhalter"},{"given":"S.","family":"Ninkovi\u0107"},{"given":"A.","family":"Cingel"},{"given":"D.","family":"Vinterhalter"}],"issued":{"date-parts":["2006"]},"container-title":"Biologia Plantarum","volume":"50","issue":"4","page":"767\u2013770"}]

[{"id":770,"citationNumber":"21.","type":"article-journal","title":"Hairy roots formation in recalcitrant-to-transform plant Chenopodium rubrum","DOI":null,"author":[{"given":"S.","family":"Dmitrovi\u0107"},{"given":"N.","family":"Miti\u0107"},{"given":"S.","family":"Zdravkovi\u0107-Kora\u0107"},{"given":"B.","family":"Vinterhalter"},{"given":"S.","family":"Ninkovi\u0107"},{"given":"L.J.","family":"\u0106ulafi\u0107"}],"issued":{"date-parts":["2010"]},"container-title":"Biologia Plantarum","volume":"54","issue":"3","page":"566\u2013570"}]

[{"id":771,"citationNumber":"22.","type":"article-journal","title":"Variability in shoot cultures regenerated from hairy roots of Gentiana punctata","DOI":null,"author":[{"given":"B.","family":"Vinterhalter"},{"given":"S.","family":"Zdravkovi\u0107-Kora\u0107"},{"given":"S.","family":"Ninkovi\u0107"},{"given":"N.","family":"Miti\u0107"},{"given":"T.","family":"Jankovi\u0107"},{"given":"J.","family":"Milju\u0161-Djuki\u0107"},{"given":"D.","family":"Vinterhalter"}],"issued":{"date-parts":["2011"]},"container-title":"Biologia Plantarum","volume":"55","issue":"3","page":"414\u2013 422"}]

[{"id":772,"citationNumber":"23.","type":"article-journal","title":"Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri T-DNA gene integration","DOI":null,"author":[{"given":"J.","family":"Batra"},{"given":"A.","family":"Dutta"},{"given":"D.","family":"Singh"},{"given":"S.","family":"Kumar"},{"given":"J.","family":"Sen"}],"issued":{"date-parts":["2004"]},"container-title":"Plant Cell Reports","volume":"23","issue":"3","page":"148\u2013154 66"}]

[{"id":773,"citationNumber":"24.","type":"article-journal","title":"Silver birch (Betula pendula plants with aux and rol genes show consistent changes in morphology, xylem structure and chemistry","DOI":null,"author":[{"given":"R.","family":"Piispanen"},{"given":"T.","family":"Aronen"},{"given":"X.","family":"Chen"},{"given":"P.","family":"Saranp\u00e4\u00e4"},{"given":"H.","family":"H\u00e4ggman"}],"issued":{"date-parts":["2003"]},"container-title":"Tree Physiology","volume":"23","issue":"11","page":"721\u2013733"}]

[{"id":774,"citationNumber":"25.","type":"article-journal","title":"Caffeic acid derivatives from a hairy root culture of Lactuca virosa","DOI":null,"author":[{"given":"A.","family":"Stojakowska"},{"given":"J.","family":"Malarz"},{"given":"A.","family":"Szewczyk"},{"given":"W.","family":"Kisiel"}],"issued":{"date-parts":["2012"]},"container-title":"Acta Physiologiae Plantarum","volume":"34","issue":"1","page":"291\u2013298"}]

[{"id":775,"citationNumber":"26.","type":"journal-article","title":"Production of caffeic, chlorogenic and rosmarinic acids in plants and suspension cultures of Glechoma hederacea","DOI":"10.1016\/j.phytol.2014.05.012","author":[{"given":"Anne Sarah","family":"D\u00f6ring"},{"given":"Maike","family":"Petersen"}],"issued":{"date-parts":["2014"]},"container-title":null,"volume":"10","issue":null,"page":"cxi-cxvii"}]

[{"id":776,"citationNumber":"27.","type":"article-journal","title":"Chlorogenic acid in a Nicotiana plumbaginifolia cell suspension","DOI":null,"author":[{"given":"F.","family":"Gillet"},{"given":"F.","family":"Mesnard"},{"given":"O.","family":"Fliniaux"},{"given":"J.-P.","family":"Monti"},{"given":"M.-A.","family":"Fliniaux"}],"issued":{"date-parts":["1999"]},"container-title":"Plant Physiology and Biochemistry","volume":"37","issue":"11","page":"869\u2013874"}]

[{"id":777,"citationNumber":"28.","type":"article-journal","title":"Accumulation of rosmarinic, chlorogenic and caffeic acids in in vitro cultures of Eryngium planum L","DOI":null,"author":[{"given":"M.","family":"Kikowska"},{"given":"J.","family":"Budzianowski"},{"given":"A.","family":"Krawczyk"},{"given":"B.","family":"Thiem"}],"issued":{"date-parts":["2012"]},"container-title":"Acta Physiologiae Plantarum","volume":"34","issue":"6","page":"2425\u20132433"}]

[{"id":778,"citationNumber":"29.","type":"article-journal","title":"Enhanced production of caftaric acid, chlorogenic acid and cichoric acid in suspension cultures of Echinacea purpurea by the manipulation of incubation temperature and photoperiod","DOI":null,"author":[{"given":"C.-H.","family":"Wu"},{"given":"H.N.","family":"Murthy"},{"given":"E.-J.","family":"Hahn"},{"given":"K.-Y.","family":"Paek"}],"issued":{"date-parts":["2007"]},"container-title":"Biochemical Engineering Journal","volume":"36","issue":"3","page":"301\u2013303"}]

[{"id":779,"citationNumber":"30.","type":"article-journal","title":"Transgenic crops for improved pharmaceutical products","DOI":null,"author":[{"given":"K.-M.","family":"Oksman-Caldentey"},{"given":"R.","family":"Hiltunen"}],"issued":{"date-parts":["1996"]},"container-title":"Field Crops Research","volume":"45","issue":"1\u20133","page":"57\u201369"}]

[{"id":780,"citationNumber":"31.","type":"article-journal","title":"Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes","DOI":null,"author":[{"given":"K.","family":"Chaudhuri"},{"given":"B.","family":"Ghosh"},{"given":"D.","family":"Tepfer"},{"given":"S.","family":"Jha"}],"issued":{"date-parts":["2006"]},"container-title":"Plant Cell Reports","volume":"25","issue":"10","page":"1059\u20131066"}]

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[{"id":782,"citationNumber":"33.","type":"article-journal","title":"Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones","DOI":null,"author":[{"given":"J.","family":"Koperd\u00e1kov\u00e1"},{"given":"H.","family":"Komarovsk\u00e1"},{"given":"J.","family":"Ko\u0161uth"},{"given":"A.","family":"Giovannini"},{"given":"E.","family":"\u010cell\u00e1rov\u00e1"}],"issued":{"date-parts":["2009"]},"container-title":"Acta Physiologiae Plantarum","volume":"31","issue":"2","page":"351\u2013358"}]

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