×
Home Current Archive Editorial board
News Contact
Originalan naučni rad– Original scientific paper

DETERMINATION OF AUCUBIN BY HPLC AND HPTLC METHODS IN METHANOLIC EXTRACTS OF Veronica montana, Veronica beccabunga AND Veronica polita

By
Jelena Živković ,
Jelena Živković

Institute for Medicinal Plant Research ‘’Dr. Josif Pančić’’, Belgrade, Serbia

Teodora Janković ,
Teodora Janković

Department of Pharmacognosy, School of Pharmacy, University of Belgrade, Belgrade, Serbia

Zoran Maksimović
Zoran Maksimović

Department of Pharmacognosy, School of Pharmacy, University of Belgrade, Belgrade, Serbia

Abstract

In the present study, methanolic extracts of three Veronica species, namely Veronica montana, Veronica beccabunga and Veronica polita, were analyzed for the total iridoids, total phenylethanoids and total phenolics contents. The results showed that iridoid glycosides are the major class of tested secondary metabolites in selected Veronica species. The highest iridoid content (467.33 mg/g d.w.) was recorded in extract of V. polita, while the richest source of total phenolics and total phenylethanoids was the extract of V. montana (105.24 mg GAE/g d.w. and 16.03 mg acteoside/g d.w., respectively). Additionally, aucubin determination in tested extracts was performed by high-performance liquid chromatography (HPLC) and high-performance thin layer chromatography (HPTLC). The aucubin content in investigated samples determined by HPLC varied between 5.66 in extract of V. beccabunga and 69.96 mg/g d.w. in extract of V. polita. No significant differences were observed between the aucubin content in plant extracts obtained by HPLC and HPTLC techniques. The proposed HPTLC method is simple, rapid and more economical as compared to HPLC method, and thus it may be suitable for routine identification and quantification of aucubin in Veronica species.

References

[{"id":532,"citationNumber":"1.","type":"article-journal","title":"An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II","DOI":null,"author":[{"given":"Angiosperm Phylogeny","family":"Group"}],"issued":{"date-parts":["2003"]},"container-title":"Botanical Journal of the Linnean Society","volume":"141","issue":null,"page":"399\u2013436"}]

[{"id":533,"citationNumber":"2.","type":"article-journal","title":"Chemotaxonomy of Veroniceae and its allies in the Plantaginaceae","DOI":null,"author":[{"given":"R.M.","family":"Taskova"},{"given":"C.H.","family":"Gotfredsen"},{"given":"S.R.","family":"Jensen"}],"issued":{"date-parts":["2006"]},"container-title":"Phytochemistry","volume":"67","issue":null,"page":"286\u2013 301"}]

[{"id":534,"citationNumber":"3.","type":"article-journal","title":"Flavonoid profiles in the Heliohebe group of New Zealand Veronica (Plantaginaceae","DOI":null,"author":[{"given":"R.M.","family":"Taskova"},{"given":"T.","family":"Kokubuna"},{"given":"R.J.","family":"Grayer"},{"given":"K.G.","family":"Ryan"},{"given":"P.J.","family":"Garnock-Jones"}],"issued":{"date-parts":["2008"]},"container-title":"Biochemical Systematics and Ecology","volume":"36","issue":null,"page":"110\u2013116"}]

[{"id":535,"citationNumber":"4.","type":"article-journal","title":"Acylated flavonoids and phenol glycosides from Veronica thymoides subsp","DOI":null,"author":[{"given":"I.","family":"Saracoglu"},{"given":"M.","family":"Varel"},{"given":"S.","family":"Harput"},{"given":"A.","family":"Nagatsu"}],"issued":{"date-parts":["2004"]},"container-title":"pseudocinera, Phytochemistry","volume":"65","issue":null,"page":"2379\u20132385"}]

[{"id":536,"citationNumber":"5.","type":"article-journal","title":"Veronica: Acylated flavone glycosides as chemosystematics markers","DOI":null,"author":[{"given":"D.C.","family":"Albach"},{"given":"R.J.","family":"Grayer"},{"given":"G.C.","family":"Kite"},{"given":"S.R.","family":"Jensen"}],"issued":{"date-parts":["2005"]},"container-title":"Biochemical Systematic and Ecology","volume":"33","issue":null,"page":"1167\u20131177"}]

[{"id":537,"citationNumber":"6.","type":"article-journal","title":"Veronica: Iridoids and cornoside as chemosystematic markers","DOI":null,"author":[{"given":"S.R.","family":"Jensen"},{"given":"D.C.","family":"Albach"},{"given":"T.","family":"Ohno"},{"given":"R.J.","family":"Grayer"}],"issued":{"date-parts":["2005"]},"container-title":"Biochemical Systematic and Ecology","volume":"33","issue":null,"page":"1031\u20131047"}]

[{"id":538,"citationNumber":"7.","type":"article-journal","title":"A chemosystematic study of Veronica: Iridoid glucosides","DOI":null,"author":[{"given":"R.","family":"Grayer-Barkmeijer"}],"issued":{"date-parts":["1973"]},"container-title":"Biochemical Systematic and Ecology","volume":"1","issue":null,"page":"101\u2013110"}]

[{"id":539,"citationNumber":"8.","type":"book","title":"Biohemijska i molekularna sistematika biljaka","DOI":null,"author":[{"given":"P.D.","family":"Marin"}],"issued":{"date-parts":["2003"]},"container-title":null,"volume":null,"issue":null,"page":null}]

[{"id":540,"citationNumber":"9.","type":"article-journal","title":"Naturally occuring iridoids","DOI":null,"author":[{"given":"B.","family":"Dinda"},{"given":"S.","family":"Debnath"},{"given":"Y.","family":"Harigaya"}],"issued":{"date-parts":["2007"]},"container-title":"Chemical and Pharmaceutical Bulletin","volume":"1","issue":"2","page":"159\u2013222"}]

[{"id":541,"citationNumber":"10.","type":"article-journal","title":"Cytotoxicactivities of Stachys species","DOI":null,"author":[{"given":"E.","family":"Haznagy-Radnai"},{"given":"B.","family":"Rethy"},{"given":"S.","family":"Czigle"},{"given":"I.","family":"Zupko"},{"given":"E.","family":"Weber"},{"given":"T.","family":"Martinek"},{"given":"G.","family":"Falkay"},{"given":"I.","family":"Mathe"}],"issued":{"date-parts":["2008"]},"container-title":"Fitoterapia","volume":"79","issue":null,"page":"595\u2013597 60"}]

[{"id":542,"citationNumber":"11.","type":"article-journal","title":"Inhibition of TNF-\u03b1 and IL-6 production by aucubin through blockade of NF-\u03baB activation in RBL-2H3 mast cells","DOI":null,"author":[{"given":"H.J.","family":"Jeong"},{"given":"H.N.","family":"Koo"},{"given":"H.J.","family":"Na"},{"given":"M.S.","family":"Kim"},{"given":"S.H.","family":"Hong"},{"given":"J.W.","family":"Eom"},{"given":"K.S.","family":"Kim"},{"given":"T.Y.","family":"Shin"},{"given":"H.M.","family":"Kim"}],"issued":{"date-parts":["2002"]},"container-title":"Cytokine","volume":"18","issue":null,"page":"252\u2013259"}]

[{"id":543,"citationNumber":"12.","type":"article-journal","title":"In vitro antispasmodic activity of peracetylated penstemonoside, aucubin, and catalpol","DOI":null,"author":[{"given":"A.V.Ortiz","family":"Urbina"},{"given":"M.L.","family":"Martin"},{"given":"B.","family":"Fernandez"},{"given":"L.San","family":"Roman"},{"given":"L.","family":"Cubillo"}],"issued":{"date-parts":["1994"]},"container-title":"Planta Medica","volume":"60","issue":null,"page":"512\u2013515"}]

[{"id":544,"citationNumber":"13.","type":"article-journal","title":"Structural considerations on the iridoids as anti-inflammatory agents","DOI":null,"author":[{"given":"M.C.","family":"Recio"},{"given":"R.M.","family":"Giner"},{"given":"S.","family":"Manez"},{"given":"J.L.","family":"Rios"}],"issued":{"date-parts":["1993"]},"container-title":"Planta Medica","volume":"60","issue":null,"page":"232\u2013234"}]

[{"id":545,"citationNumber":"14.","type":"article-journal","title":"Antioxidant activity and total phenolics in selected fruits, vegetables and grain products","DOI":null,"author":[{"given":"Y.S.","family":"Velioglu"},{"given":"G.","family":"Mazza"},{"given":"L.","family":"Gao"},{"given":"B.D.","family":"Oomah"}],"issued":{"date-parts":["1998"]},"container-title":"Journal of Agriculture and Food Chemistry","volume":"46","issue":null,"page":"4113\u20134117"}]

[{"id":546,"citationNumber":"15.","type":"article-journal","title":"Council of Europe","DOI":null,"author":[{"given":"European Pharmacopoeia ","family":""}],"issued":{"date-parts":["2008"]},"container-title":"Strasbourg Cedex","volume":"6","issue":"0","page":"1307-1308"}]

[{"id":547,"citationNumber":"16.","type":"article-journal","title":"The preparation and properties of aucubin, asperuloside and some related glycosides","DOI":null,"author":[{"given":"A.R.","family":"Trimm"},{"given":"R.","family":"Hill"}],"issued":{"date-parts":["1952"]},"container-title":"Biochemical Journal","volume":"50","issue":null,"page":"310\u2013 319"}]

[{"id":548,"citationNumber":"17.","type":"chapter","title":"","DOI":null,"author":[{"given":"M.","family":"Waksmundzka-Hajnos"},{"given":"J.","family":"Sherma"}],"issued":{"date-parts":["2011"]},"container-title":"High Performance Liquid Chromatography in Phytochemical Analysis","volume":null,"issue":null,"page":null}]

[{"id":549,"citationNumber":"18.","type":"article-journal","title":"LC\/MS analysis of aucubin and catalpol of some Veronica species","DOI":null,"author":[{"given":"G.","family":"Cri\u015fan"},{"given":"L.","family":"Vlase"},{"given":"G.","family":"Balica"},{"given":"D.","family":"Muntean"},{"given":"C.","family":"\u015etef\u0103nescu"},{"given":"R.","family":"P\u0103ltinean"},{"given":"M.","family":"T\u0103mas"},{"given":"S.","family":"Leucuta"}],"issued":{"date-parts":["2010"]},"container-title":"Farmacia","volume":"58","issue":"2","page":"237\u2013242"}]

[{"id":550,"citationNumber":"19.","type":"article-journal","title":"Determination of iridoid glycosides by micellar electrokinetic capillary chromatography-mass spectrometry with use of the partial filling technique","DOI":null,"author":[{"given":"J.","family":"Suomi"},{"given":"S.K.","family":"Wiedmer"},{"given":"M.","family":"Jussila"},{"given":"M.-L.","family":"Riekkola"}],"issued":{"date-parts":["2001"]},"container-title":"Electrophoresis","volume":"22, (12","issue":null,"page":"2580\u20132587"}]

[{"id":551,"citationNumber":"20.","type":"chapter","title":"","DOI":null,"author":[{"given":"M.M.","family":"Srivastava"}],"issued":{"date-parts":["2011"]},"container-title":"High-Performance Thin-Layer Chromatography (HPTLC","volume":null,"issue":null,"page":null}]

[{"id":552,"citationNumber":"21.","type":"article-journal","title":"Quantitative determination of aucubin in seven Plantago species using HPLC, HPTLC and LC-ESI-MS methods","DOI":null,"author":[{"given":"T.","family":"Jankovi\u0107"},{"given":"N.","family":"Menkovi\u0107"},{"given":"G.","family":"Zduni\u0107"},{"given":"I.","family":"Beara"},{"given":"K.","family":"Balog"},{"given":"K.","family":"\u0160avikin"},{"given":"N.","family":"Mimica-Duki\u0107"}],"issued":{"date-parts":["2010"]},"container-title":"Analytical Letters","volume":"43","issue":null,"page":"2487\u20132495"}]

[{"id":553,"citationNumber":"22.","type":"article-journal","title":"Comparison between HPLC and HPTLCdensitometry for the determination of harpagoside from Harpagophytum procumbens CO2-extracts","DOI":null,"author":[{"given":"M.","family":"Gunther"},{"given":"P.C.","family":"Schmidt"}],"issued":{"date-parts":["2005"]},"container-title":"Journal of Pharmaceutical and Biomedical Analysis","volume":"37","issue":null,"page":"817\u2013821"}]

Citation

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

Article metrics

Google scholar: See link

The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.