Institute for Medicinal Plant Research ‘’Dr. Josif Pančić’’ , Belgrade , Serbia
Department of Pharmacognosy, School of Pharmacy, University of Belgrade , Belgrade , Serbia
Department of Pharmacognosy, School of Pharmacy, University of Belgrade , Belgrade , Serbia
In the present study, methanolic extracts of three Veronica species, namely Veronica montana, Veronica beccabunga and Veronica polita, were analyzed for the total iridoids, total phenylethanoids and total phenolics contents. The results showed that iridoid glycosides are the major class of tested secondary metabolites in selected Veronica species. The highest iridoid content (467.33 mg/g d.w.) was recorded in extract of V. polita, while the richest source of total phenolics and total phenylethanoids was the extract of V. montana (105.24 mg GAE/g d.w. and 16.03 mg acteoside/g d.w., respectively). Additionally, aucubin determination in tested extracts was performed by high-performance liquid chromatography (HPLC) and high-performance thin layer chromatography (HPTLC). The aucubin content in investigated samples determined by HPLC varied between 5.66 in extract of V. beccabunga and 69.96 mg/g d.w. in extract of V. polita. No significant differences were observed between the aucubin content in plant extracts obtained by HPLC and HPTLC techniques. The proposed HPTLC method is simple, rapid and more economical as compared to HPLC method, and thus it may be suitable for routine identification and quantification of aucubin in Veronica species.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.