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Diploid vs. tetraploid Centaurium erythraea Rafn: a comparative study of regenerative in vitro potential and biosynthetic capacity

By
Biljana Filipović ,
Biljana Filipović

Institute for Biological Research “Siniša Stankovic“, University of Belgrade, Belgrade, Serbia

Branislav Šiler ,
Branislav Šiler

Institute for Biological Research “Siniša Stankovic“, University of Belgrade, Belgrade, Serbia

Jasmina Nestorović-Živković ,
Jasmina Nestorović-Živković

Institute for Biological Research “Siniša Stankovic“, University of Belgrade, Belgrade, Serbia

Tijana Banjanac ,
Tijana Banjanac

Institute for Biological Research “Siniša Stankovic“, University of Belgrade, Belgrade, Serbia

Marijana Skorić ,
Marijana Skorić

Institute for Biological Research “Siniša Stankovic“, University of Belgrade, Belgrade, Serbia

Jelena Božunović ,
Jelena Božunović

Institute for Biological Research “Siniša Stankovic“, University of Belgrade, Belgrade, Serbia

Danijela Mišić
Danijela Mišić

Institute for Biological Research “Siniša Stankovic“, University of Belgrade, Belgrade, Serbia

Abstract

The impact of ploidy level on both the regenerative potential under in vitro conditions and the production of major bioactive specialized metabolites, such are iridoids and xanthones, was examined in Centaurium erythraea Rafn. Shoot regeneration frequency was genotype dependent, but not affected by explant ploidy level. In most cases, the regenerated shoots of autotetraploid (4x) genotypes were more robust than diploid (2x) ones. Regeneration efficiency of root explants declined from the apical to the basal root segment. Shoot and root biomass production of two month-old shoots was not significantly different between 2x and 4x genotypes. Both 4x and 2x genotypes were characterized by the predominance of secoiridoid glucoside gentiopicrin in shoots and roots, which is followed by sweroside and swertiamarin. Loganic acid, loganin and secologanin were much less abundant. Methylbellidifolin was the major xanthone in both shoots and roots. Diploid plants showed higher biosynthetic capacity for the production of secoiridoids and xanthones in both shoots and roots. Results highlight a higher potential of diploid C. erythraea genotypes for biotechnology-based sustainable production of secoiridoids in comparison to tetraploid genotypes.


 

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