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Originalan naučni rad– Original scientific paper

NON-TUMOR/TUMOR IC50 RATIO AS PARAMETER FOR EVALUATION OF IN VITRO ANTITUMOR ACTIVITY OF THE PLANTAGO, ALLIUM AND TUBER SPECIES

By
Dragana Četojević-Simin ,
Dragana Četojević-Simin

Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia

Ivana Beara ,
Ivana Beara

Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia

Nataša Simin
Nataša Simin

Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Novi Sad, Serbia

Abstract

The goal of this work was to introduce the parameter for the precise evaluation of in vitroanti-tumor activity of Plantago altissima, P. lanceolata, P. reniformis, Allium flavum, A. cepa, Tuber aestivum i T. magnatumextracts.For the evaluation of the cell growth human tumor cell lines HeLa, MCF7 and HT-29 were used, as well as cell line derived from the healthy tissue (MRC-5). The cell growth was evaluated using colorimetric sulforhodamine B (SRB) assay.NT/T IC50 parameter gives precise information on the in vitro anti-tumor efficiency of evaluated extracts. The highest efficiency was achieved using water extracts of black and white truffle towards breast adenocarcinoma , reaching extremely high NT/T values (NT/T=200-500). The efficiency of several methanolic extracts of truffles was high (NT/T=21,33-70): while the efficiency of all Plantago sp.extracts was moderate (NT/T=3,24-4,27) towards breast adenocarcinoda and cervix carcinoma. The least efficient were extracts of aerial parts of A. flavum that have been more cytotoxic towards healthy cells than to colon adenocarcinoma (NT/T=0,23) and cervix carcinoma (NT/T=0,75).Extremely high NT/T valus that were obtained using water extracts of black and white truffle recommend their use in the anti-tumor therapy and suggest their use in health prevention and in the production of nutraceuticals and food supplements.


 

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