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Institute for the Application of Nuclear Energy INEP, University of Belgrade, Banatska 31b , 11080 Belgrade , Serbia
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Institute of Chemistry, Technology, and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade, Njegoševa 12 , 11 000 Belgrade , Serbia
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Institute of Chemistry, Technology, and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade, Njegoševa 12 , 11 000 Belgrade , Serbia
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4 , 11 000 Belgrade , Serbia
With the aim to examine the radical scavenging activity of smoke tree extract-loaded liposomal particles before and after ultrasound exposure (45 or 70% amplitude and 15 or 30 min), ABTS and DPPH tests were employed. The antioxidant activity of the pure extract was 11.37±0.52 µmol Trolox equivalent (TE)/mL and 79.7±0.5%. Multilamellar liposomes with extract showed significantly higher antioxidant activity in both assays (12.02±0.54 µmol TE/mL and 81.9±0.4%) compared to sonicated liposomes (10.75-11.00 µmol TE/mL and 79.3-80.9%) and pure extract. There was no significant difference between the ABTS radical scavenging activity of the liposomes treated by different amplitudes and times, while prolonged sonication and a higher amplitude caused a significant drop in the anti-DPPH capacity of extract-loaded liposomal vesicles. The presented results and the differences between the obtained data provide a good insight into the overall antioxidant capacity of smoke tree extract-loaded multilamellar and sonicated unilamellar liposomal vesicles.
This work was supported by the Ministry of Science, Technological Development and Innovation of Serbia (451-03-66/2024-03/200019, 451-03-66/2024-03/200135, and 451-03-66/2024-03/200026).
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