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Institute for Medicinal Plants Research „Dr. Josif Pančić“, Tadeuša Košćuška 1 , 11 000 Belgrade , Serbia
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Institute for Medicinal Plants Research „Dr. Josif Pančić“, Tadeuša Košćuška 1 , 11 000 Belgrade , Serbia
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Institute for Medicinal Plants Research „Dr. Josif Pančić“, Tadeuša Košćuška 1 , 11 000 Belgrade , Serbia
Institute for Medicinal Plants Research „Dr. Josif Pančić“, Tadeuša Košćuška 1 , 11 000 Belgrade , Serbia
As a rich source of polyphenolic compounds, Fagopyrum esculentum Moench herb, is a valuable subject for a numerous industries, such as pharmaceutical, chemical, food, or cosmetic. Given the biological potential that is closely related to the polyphenolic content, this study aimed to develop ecologically suitable cyclodextrin-aided extraction from the aerial parts of F. esculentum. To maximize the polyphenolic content, highly-reliable mathematical and statistical method – response surface methodology (RSM) was used for the evaluation of crucial extraction parameters: temperature, extraction time, concentrations of ethanol and two types of cyclodextrins (β-cyclodextrin (β-CD), and (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD)). While temperature and ethanol concentration significantly influenced the total phenolic content (TPC) in both models, the impact of cyclodextrin concentration was observed when HP-β-CD was used. According to the RSM analysis, optimal conditions for the maximization of TPC were noted for the extraction at 80 °C for 40 min, using 50% ethanol and 1.50% HP-β-CD. Thus, under optimized extraction conditions, the measured TPC was 42.1562 ± 1.0101 mg GAE/g DW, among rutin was the most abundant with content of 29.5479 mg/g DW, followed by the quercetin and quercitrin in slightly scarcer content. This study confirmed that cyclodextrin-aided extraction is an ecologically friendly, effective method for the recovery of F. esculentum polyphenolics.
This research was supported by the Ministry of Science, Technological Development and Innovation of the Republic of Serbia, grant number 451-03-66/2024-03/200003.
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