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Sensitivity of multiresistant bacteria and methicillin-resistant Staphylococcus aureus to ethanolic root extract of Raphanus sativus

By
Dejan Stojković ,
Dejan Stojković

Department of Plant Physiology, Institute for Biological Research “Siniša Stankovic”, University of Belgrade, Belgrade, Serbia

Marija Smiljković ,
Marija Smiljković

Department of Plant Physiology, Institute for Biological Research “Siniša Stankovic”, University of Belgrade, Belgrade, Serbia

Miloš Nikolić ,
Miloš Nikolić

Department of Plant Physiology, Institute for Biological Research “Siniša Stankovic”, University of Belgrade, Belgrade, Serbia

Jelena Živković ,
Jelena Živković
Contact Jelena Živković

Institute for Medicinal Plant Research “Dr. Josif Pančić”, Belgrade, Serbia

Marina Soković
Marina Soković

Department of Plant Physiology, Institute for Biological Research “Siniša Stankovic”, University of Belgrade, Belgrade, Serbia

Abstract

Raphanus sativus L. (Brassicaceae) is an edible plant, whose root is consumed all over the world. The objective of this study was to test antibacterial potential of R. sativus ethanolic extract on 12 pathogenic bacteria including multiresistant bacterial strains and MRSA. All of the tested bacteria showed sensitivity to the antibacterial effect of R. sativus ethanolic extract with minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) in range of 15µg/mL – 500µg/mL. It is interesting to note that raphanus extract showed the highest activity against multiresistant strain of Pseudomonas aeruginosa with equal inhibitory and bactericidal concentrations of 15µg/mL, while the most resistant strain to the effect of the extract was Proteus mirabilis (MIC and MBC of the extract were 300 µg/mL and 500µg/mL, respectively). In disk diffusion assay, zones of inhibitions were measured ranging from 8 mm to 22 mm.


 

References

[{"id":2490,"citationNumber":"1.","type":"article-journal","title":"The genera of Brassiceae (Cruciferae; Brassicaceae) in the southeastern United States","DOI":null,"author":[{"given":"I.","family":"Al-Shehbaz"}],"issued":{"date-parts":["1985"]},"container-title":"Journal of the Arnold Arboretum","volume":"66","issue":null,"page":"279\u2013351"}]

[{"id":2491,"citationNumber":"2.","type":"article-journal","title":"How to manage Pseudomonas aeruginosa infections","DOI":null,"author":[{"given":"M.","family":"Bassetti"},{"given":"A.","family":"Vena"},{"given":"A.","family":"Croxatto"},{"given":"E.","family":"Righi"},{"given":"B.","family":"Guery"}],"issued":{"date-parts":["2018"]},"container-title":"Drugs in Context","volume":"7","issue":null,"page":"212527"}]

[{"id":2492,"citationNumber":"3.","type":"article-journal","title":"Isothiocyanate Profile and Selective Antibacterial Activity of Root, Stem, and Leaf Extracts Derived from Raphanus sativus L","DOI":null,"author":[{"given":"S.S.","family":"Beevi"},{"given":"L.N.","family":"Mangamoori"},{"given":"V.","family":"Dhand"},{"given":"D.S.","family":"Ramakrishna"}],"issued":{"date-parts":["2009"]},"container-title":"Foodborne Pathogens and Disease","volume":"6","issue":"1","page":"129\u2013136"}]

[{"id":2493,"citationNumber":"4.","type":"article-journal","title":"Antilithiasic and Hypolipidaemic Effects of Raphanus sativus L. var. niger on Mice Fed with a Lithogenic Diet","DOI":null,"author":[{"given":"I.G.","family":"Castro-Torres"},{"given":"E.B.","family":"Naranjo-Rodriguez"},{"given":"M.","family":"\u00c1ngel Dominguez-Ortiz"},{"given":"J.","family":"Gallegos-Estudillo"},{"given":"M.V.","family":"SaavedraVelez"}],"issued":{"date-parts":["2012"]},"container-title":"Journal of Biomedicine and Biotechnology","volume":"2012","issue":null,"page":"1\u20138"}]

[{"id":2494,"citationNumber":"5.","type":"book","title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically","DOI":null,"author":[{"given":"C.L.S.I.","family":""}],"issued":{"date-parts":["2009"]},"container-title":null,"volume":null,"issue":null,"page":null}]

[{"id":2495,"citationNumber":"6.","type":"article-journal","title":"In vitro probiotic characterization of Lactobacillus strains from fermented radish and their anti-adherence activity against enteric pathogens","DOI":null,"author":[{"given":"K.","family":"Damodharan"},{"given":"S.A.","family":"Palaniyandi"},{"given":"S.H.","family":"Yang"},{"given":"J.-W.","family":"Suh"}],"issued":{"date-parts":["2015"]},"container-title":"Canadian Journal of Microbiology","volume":"61","issue":"11","page":"837\u2013850"}]

[{"id":2496,"citationNumber":"7.","type":"book","title":"The Oxford Companion to Food","DOI":null,"author":[{"given":"A.","family":"Davidson"}],"issued":{"date-parts":["2014"]},"container-title":null,"volume":null,"issue":null,"page":null}]

[{"id":2497,"citationNumber":"8.","type":"article-journal","title":"Medicinal plants of China","DOI":null,"author":[{"given":"J.A.","family":"Duke"},{"given":"E.S.","family":"Ayensu"}],"issued":{"date-parts":["1985"]},"container-title":null,"volume":null,"issue":null,"page":null}]

[{"id":2498,"citationNumber":"9.","type":"article-journal","title":"Raphanus sativus (Radish): Their Chemistry and Biology","DOI":null,"author":[{"given":"R.M.P.","family":"Gutierrez"},{"given":"R.L.","family":"Perez"}],"issued":{"date-parts":["2004"]},"container-title":"The Scientific World Journal","volume":"4","issue":null,"page":"811\u2013837"}]

[{"id":2499,"citationNumber":"10.","type":"article-journal","title":"Variation in amylase activities in radish (Raphanus sativus) cultivars, Plant Foods for Human Nutrition","DOI":null,"author":[{"given":"M.","family":"Hara"},{"given":"F.","family":"Ito"},{"given":"T.","family":"Asai"},{"given":"T.","family":"Kuboi"}],"issued":{"date-parts":["2009"]},"container-title":null,"volume":"64","issue":"3","page":"188\u2013 192"}]

[{"id":2500,"citationNumber":"11.","type":"book","title":"The New Royal Horticultural Society dictionary of gardening","DOI":null,"author":[{"given":"A.","family":"Huxley"},{"given":"M.","family":"Griffiths"},{"given":"M.","family":"Levy"}],"issued":{"date-parts":["1992"]},"container-title":null,"volume":"2","issue":null,"page":null}]

[{"id":2501,"citationNumber":"12.","type":"article-journal","title":"Design, synthesis and antimicrobial activity of usnic acid derivatives","DOI":null,"author":[{"given":"V.","family":"Kartsev"},{"given":"B.","family":"Lichitsky"},{"given":"A.","family":"Geronikaki"},{"given":"A.","family":"Petrou"},{"given":"M.","family":"Smiljkovic"},{"given":"M.","family":"Kostic"},{"given":"O.","family":"Radanovic"},{"given":"M.","family":"Sokovic"}],"issued":{"date-parts":["2018"]},"container-title":"MedChemComm","volume":"9","issue":"5","page":"870\u2013882"}]

[{"id":2502,"citationNumber":"13.","type":"book","title":"Mabberley\u2019s Plant-book: A Portable Dictionary of Plants, their Classification and Uses","DOI":null,"author":[{"given":"D.J.","family":"Mabberley"}],"issued":{"date-parts":["2008"]},"container-title":null,"volume":null,"issue":null,"page":null}]

[{"id":2503,"citationNumber":"14.Mithen,","type":"article-journal","title":"Glucosinolate biochemical diversity and innovation in the Brassicales","DOI":null,"author":[{"given":"Bennett","family":"R."},{"given":"R.","family":""},{"given":"J.","family":"Marquez"}],"issued":{"date-parts":["2010"]},"container-title":"Phytochemistry","volume":"71","issue":"17-18","page":"2074\u20132086"}]

[{"id":2504,"citationNumber":"15.","type":"article-journal","title":"4- (Methylthio)-3-butenyl Isothiocyanate, a Principal Antimutagen in Daikon (Raphanus sativus; Japanese White Radish","DOI":null,"author":[{"given":"Y.","family":"Nakamura"},{"given":"T.","family":"Iwahashi"},{"given":"A.","family":"Tanaka"},{"given":"J.","family":"Koutani"},{"given":"T.","family":"Matsuo"},{"given":"S.","family":"Okamoto"},{"given":"K.","family":"Sato"},{"given":"K.","family":"Ohtsuki"}],"issued":{"date-parts":["2001"]},"container-title":"Journal of Agricultural and Food Chemistry","volume":"49","issue":"12","page":"5755\u20135760"}]

[{"id":2505,"citationNumber":"16.","type":"article-journal","title":"Antimicrobial and antioxidant properties of the flavonoid extract from Raphanus sativus L","DOI":null,"author":[{"given":"P.T.K.","family":"Ngoc"},{"given":"N.T.M.","family":"Nguyet"},{"given":"D.T.A.","family":"Dao"}],"issued":{"date-parts":["2017"]},"container-title":"AIP Conference Proceedings","volume":"1878","issue":"1","page":"020026"}]

[{"id":2506,"citationNumber":"17.","type":"article-journal","title":"Estonian folk traditional experiences on natural anticancer remedies: From past to the future","DOI":null,"author":[{"given":"K.","family":"Sak"},{"given":"K.","family":"Jurisoo"},{"given":"A.","family":"Raal"}],"issued":{"date-parts":["2014"]},"container-title":"Pharmaceutical Biology","volume":"52","issue":"7","page":"855\u2013866"}]

[{"id":2507,"citationNumber":"18.","type":"article-journal","title":"Antimicrobial Activity of Essential Oils Isolated from Different Parts of Endemic Plant Portenschlagiella ramosissima Tutin","DOI":null,"author":[{"given":"M.D.","family":"Sokovic"},{"given":"J.","family":"Glamoclija"},{"given":"A.","family":"Ciric"},{"given":"D.","family":"Grubi\u0161ic"},{"given":"D.","family":"Stojkovic"},{"given":"M.","family":"Ristic"}],"issued":{"date-parts":["2008"]},"container-title":"Journal of Essential Oil Research","volume":"20","issue":"4","page":"369\u2013372"}]

[{"id":2508,"citationNumber":"19.","type":"article-journal","title":"Precision antimicrobial therapeutics: the path of least resistance?","DOI":null,"author":[{"given":"C.N.","family":"Spaulding"},{"given":"R.D.","family":"Klein"},{"given":"H.L.","family":"Schreiber"},{"given":"J.W.","family":"Janetka"},{"given":"S.J.","family":"Hultgren"}],"issued":{"date-parts":["2018"]},"container-title":"npj Biofilms and Microbiomes","volume":"4","issue":null,"page":"4 1\u20137"}]

[{"id":2509,"citationNumber":"20.","type":"article-journal","title":"Methicillin resistance in Staphylococcus aureus: mechanisms and modulation","DOI":null,"author":[{"given":"P.D.","family":"Stapleton"},{"given":"P.W.","family":"Taylor"}],"issued":{"date-parts":["2002"]},"container-title":"Science Progress","volume":"85","issue":"1","page":"57\u201372"}]

[{"id":2510,"citationNumber":"21.","type":"book","title":"Handbook of Chinese Herbal Formulas","DOI":null,"author":[{"given":"H.C.","family":"Yeung"}],"issued":{"date-parts":["1985"]},"container-title":null,"volume":null,"issue":null,"page":null}]

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