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Inhibition of quorum sensing virulente factors of Pseudomonas aeruginosa PAO1 by Ferulago macedonica and Echinophora sibthorpiana extracts and essential oils

By
Ksenija Mileski ,
Ksenija Mileski
Contact Ksenija Mileski

Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Ana Ćirić ,
Ana Ćirić

Dep. of Plant Physiology, Institute for Biological Research “Siniša Stankovic”, University of Belgrade, Belgrade, Serbia

Vlado Matevski ,
Vlado Matevski

Institute of Biology, Faculty of Natural Sciences and Mathematics, University “S. Kiril and Metodij”, Skopje,

Macedonian Academy of Sciences and Arts, Skopje,

Petar Marin ,
Petar Marin

Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Marina Soković ,
Marina Soković

Dep. of Plant Physiology, Institute for Biological Research “Siniša Stankovic”, University of Belgrade, Belgrade, Serbia

Ana Džamić
Ana Džamić

Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Abstract

This study was conducted to analyse extracts and essential oils of Ferulago macedonica and Echinophora sibthorpiana (Apiaceae) for anti–quorum sensing potential using Pseudomonas aerugonisa PAO1 as biomonitoring system. Anti–quorum sensing screening was carried out by tree different tests which showed that examined samples possessed significant effects in suppressing of some virulent determinants of PAO1. The essential oils of F. macedonica and E. sibthorpiana aerial parts reduced the growth and modified the appearance of P. aeruginosa colonies and flagella to a large extent in twitching and motility assay. In the presence of essential oil of E. sibthorpiana, diameter of the colony was 6.67 mm, the flagella were absent and the color of the colony was changed from green to white. In anti–biofilm assay, the inhibitory activity of E. sibthorpiana was more notable than of F. macedonica. Applied colorimetric test revealed that examined samples strongly reduced the production of pigment pyocyanin where the highest inhibition of its synthesis showed F. macedonica oil (5.22%), followed by E. sibthorpiana oil (10.69%), which was singnificantly bigger potential in comparison to used antibiotics (141.55%).


 

References

[{"id":2043,"citationNumber":"1.","type":"article-journal","title":"Anti-quorum sensing agents from south Florida medicinal plants and their attenuation of Pseudomonas aeruginosa pathogenicity, ProQuest ETD Collection for FIU","DOI":null,"author":[{"given":"A.L.","family":"Adonizio"}],"issued":{"date-parts":["2008"]},"container-title":null,"volume":null,"issue":null,"page":"1\u2013171"}]

[{"id":2044,"citationNumber":"2.","type":"article-journal","title":"Analysis of the Essential Oil of Echinophora sibthorpiana Guss","DOI":null,"author":[{"given":"V.U.","family":"Ahmad"},{"given":"A.R.","family":"Jassbi"},{"given":"M.S.C.","family":"Pannahi"}],"issued":{"date-parts":["1999"]},"container-title":null,"volume":null,"issue":null,"page":null}]

[{"id":2045,"citationNumber":"3.","type":"article-journal","title":"GC\/MS and 13 C-NMR Techniques","DOI":null,"author":[{"given":"Means","family":"GC"}],"issued":{"date-parts":[null]},"container-title":"Journal of Essential Oil Research","volume":"11","issue":"1","page":"107\u2013108"}]

[{"id":2046,"citationNumber":"4.","type":"article-journal","title":"Essential Oils of Echinophora lamondiana (Apiales: Umbelliferae): A Relationship Between Chemical Profile and Biting Deterrence and Larvicidal Activity Against Mosquitoes (Diptera: Culicidae","DOI":null,"author":[{"given":"A.","family":"Ali"},{"given":"N.","family":"Tabanca"},{"given":"G.","family":"Ozek"},{"given":"T.","family":"Ozek"},{"given":"Z.","family":"Aytac"},{"given":"U.R.","family":"Bernier"},{"given":"N.M.","family":"Agramonte"},{"given":"K.H.C.","family":"Baser"},{"given":"I.A.","family":"Khan"}],"issued":{"date-parts":["2015"]},"container-title":"Journal of Medical Entomology","volume":"52","issue":"1","page":"93\u2013 100"}]

[{"id":2047,"citationNumber":"5.","type":"article-journal","title":"Antifungal Effect of Echinophora platyloba\u2019 s Extract against Candida albicans","DOI":null,"author":[{"given":"M.","family":"Avijgan"},{"given":"M.","family":"Hafizi"},{"given":"M.","family":"Saadat"},{"given":"M.A.","family":"Nilforoushzadeh"}],"issued":{"date-parts":["2010"]},"container-title":"Iranian Journal of Pharmaceutical Research","volume":"0","issue":"0","page":"285\u2013289"}]

[{"id":2048,"citationNumber":"6.","type":"article-journal","title":"The comparative study of anti-fungal effect of pharmaceutical products containing hydroalcoholic extract of Echinophora platyloba DC and flucona- zole in women with chronic recurrent vaginitis caused by candida albicans","DOI":null,"author":[{"given":"M.","family":"Avijgan"},{"given":"F.","family":"Mirzadeh"},{"given":"E.","family":"Ahmadi Nia"}],"issued":{"date-parts":["2012"]},"container-title":"Research Journal of Medical Sciences","volume":"17","issue":null,"page":"103\u2013 107"}]

[{"id":2049,"citationNumber":"7.","type":"article-journal","title":"Essential Oil of Echinophora tenuifolia L","DOI":null,"author":[{"given":"K.H.","family":"Baser"},{"given":"F.Z.","family":"Erdemgil"},{"given":"T.","family":"\u00d6zek"}],"issued":{"date-parts":["1994"]},"container-title":"subsp. sibthorpiana (Guss.) Tutin, Journal of Essential Oil Research","volume":"6","issue":"4","page":"399\u2013400"}]

[{"id":2050,"citationNumber":"8.","type":"article-journal","title":"Essential Oils of Six Echinophora Species from Turkey","DOI":null,"author":[{"given":"K.H.","family":"Baser"},{"given":"M.","family":"K\u00fcrkc\u00fcoglu"},{"given":"H.","family":"Malyer"},{"given":"A.","family":"Bicakci"}],"issued":{"date-parts":["1998"]},"container-title":"Journal of Essential Oil Research","volume":"10","issue":"3","page":"345\u2013351"}]

[{"id":2051,"citationNumber":"9.","type":"article-journal","title":"Plant responses to bacterial quorum sensing signals","DOI":null,"author":[{"given":"W.D.","family":"Bauer"},{"given":"U.","family":"Mathesius"},{"given":"U.","family":"Cakilcioglu"},{"given":"I.","family":"Turkoglu"}],"issued":{"date-parts":["2004"]},"container-title":"Current Opinion in Plant Biology","volume":"7","issue":"4","page":"429\u2013433"}]

[{"id":2052,"citationNumber":"10.","type":"chapter","title":"Ferulago W","DOI":null,"author":[{"given":"J.","family":"Canonn"}],"issued":{"date-parts":["1968"]},"container-title":"Flora Europaea","volume":null,"issue":null,"page":"359\u2013360"}]

[{"id":2053,"citationNumber":"11.","type":"article-journal","title":"Essential oil from fruits and roots of Ferulago campestris (Besser) Grecescu (Apiaceae): composition and antioxidant and antiCandida activity","DOI":null,"author":[{"given":"C.","family":"Cecchini"},{"given":"M.M.","family":"Coman"},{"given":"A.","family":"Cresci"},{"given":"B.","family":"Tirillini"},{"given":"G.","family":"Cristalli"},{"given":"F.","family":"Papa"},{"given":"G.","family":"Sagratini"},{"given":"S.","family":"Vittori"},{"given":"F.","family":"Maggi"}],"issued":{"date-parts":["2010"]},"container-title":"Flavour and Fragrance Journal","volume":"25","issue":"6","page":"493\u2013502"}]

[{"id":2054,"citationNumber":"12.","type":"article-journal","title":"Influence of Tarhana Herb (Echinophora sibthorpiana) on Fermentation of Tarhana, Turkish Traditional Fermented Food","DOI":null,"author":[{"given":"N.","family":"Degirmencioglu"},{"given":"D.","family":"Gocmen"},{"given":"A.","family":"Dagdelen"},{"given":"F.","family":"Dagdelen"}],"issued":{"date-parts":["2005"]},"container-title":"Food Technology and Biotechnology","volume":"43","issue":null,"page":"175\u2013179"}]

[{"id":2055,"citationNumber":"13.","type":"article-journal","title":"Comparing the Effects of Echinophora-platyloba, Fennel and Placebo on Pre-menstrual Syndrome","DOI":null,"author":[{"given":"M.","family":"Delaram"},{"given":"S.","family":"Kheiri"},{"given":"M.R.","family":"Hodjati"}],"issued":{"date-parts":["2011"]},"container-title":"Journal of Reproduction & Infertility","volume":"12","issue":"3","page":"221\u2013226"}]

[{"id":2056,"citationNumber":"14.","type":"article-journal","title":"Ethnobotanical studies of some apiaceae plants in kahramanmaras and a review of their phytochemical studies","DOI":null,"author":[{"given":"S.","family":"Demirci"},{"given":"E.","family":"\u00d6zkan"}],"issued":{"date-parts":["2014"]},"container-title":"Journal of Faculty Pharmacy of Istanbul University","volume":"44","issue":"2","page":"241\u2013250"}]

[{"id":2057,"citationNumber":"15.","type":"article-journal","title":"Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variation","DOI":null,"author":[{"given":"E.","family":"Drenkard"},{"given":"F.M.","family":"Ausubel"}],"issued":{"date-parts":["2002"]},"container-title":"Nature","volume":"416","issue":"6882","page":"740\u2013743"}]

[{"id":2058,"citationNumber":"16.","type":"article-journal","title":"The antiepileptic Materia Medica of Pediacus Dioscorides","DOI":null,"author":[{"given":"M.","family":"Eadie"}],"issued":{"date-parts":["2004"]},"container-title":"Journal of Clinical Neuroscience","volume":"11","issue":"7","page":"697\u2013701"}]

[{"id":2059,"citationNumber":"17.","type":"article-journal","title":"Studying the effect Echinophora platyloba extract on bacteria (Staphilococus aureus and Pseudomonas aeroginosa) and fungi (Candidia albicans, Aspergilus flavus and Aspergilus niger) in vitro","DOI":null,"author":[{"given":"M.","family":"Entezari"},{"given":"M.","family":"Hashemi"},{"given":"M.","family":"Ashki"},{"given":"S.","family":"Ebrahimian"},{"given":"M.","family":"Bayat"},{"given":"A.","family":"Saraji"},{"given":"S.","family":"Rohani"}],"issued":{"date-parts":["2009"]},"container-title":"World Journal of Medical Science","volume":"4","issue":null,"page":"89\u201390"}]

[{"id":2060,"citationNumber":"18.","type":"article-journal","title":"Exploitation of Apiaceae family essential oils as potent biopesticides and rich source of phellandrenes","DOI":null,"author":[{"given":"E.","family":"Evergetis"},{"given":"A.","family":"Michaelakis"},{"given":"S.A.","family":"Haroutounian"}],"issued":{"date-parts":["2013"]},"container-title":"Industrial Crops and Products","volume":"41","issue":null,"page":"365\u2013370"}]

[{"id":2061,"citationNumber":"19.","type":"article-journal","title":"Determination of the chemical composition and in vitro antioxidant activities of essential oil and methanol extracts of Echinophora platyloba DC","DOI":null,"author":[{"given":"M.","family":"Gholivand"},{"given":"M.","family":"Rahimi-Nasrabadi"},{"given":"E.","family":"Mehraban"},{"given":"M.","family":"Niasari"},{"given":"H.","family":"Batooli"}],"issued":{"date-parts":["2011"]},"container-title":"Natural Product Research","volume":"25","issue":"17","page":"1585\u20131595"}]

[{"id":2062,"citationNumber":"20.","type":"article-journal","title":"Chemical characterization and biological activity of Chaga (Inonotus obliquus), a medicinal \u201cmushroom\u201d","DOI":null,"author":[{"given":"J.","family":"Glamo\u0107lija"},{"given":"A.","family":"Ciri\u0107"},{"given":"M.","family":"Nikoli\u0107"},{"given":"n","family":"Fernandes"},{"given":"L.","family":"Barros"},{"given":"R.C.","family":"Calhelha"},{"given":"I.C.","family":"Ferreira"},{"given":"M.","family":"Sokovi\u0107"},{"given":"L.J.","family":"Griensven"}],"issued":{"date-parts":["2015"]},"container-title":"Journal of Ethnopharmacology","volume":"162","issue":null,"page":"323\u2013332"}]

[{"id":2063,"citationNumber":"21.","type":"article-journal","title":"Determination of Chemical Composition, Total Phenolic, Antimicrobial, and Antioxidant Activities of Echinophora tenuifolia Essential Oil","DOI":null,"author":[{"given":"I.","family":"Gokbulut"},{"given":"T.","family":"Bilenler"},{"given":"I.","family":"Karabulut"}],"issued":{"date-parts":["2013"]},"container-title":"International Journal of Food Properties","volume":"16","issue":"7","page":"1442\u2013 1451"}]

[{"id":2064,"citationNumber":"22.","type":"article-journal","title":"Effects of yeast, fermentation time, and preservation methods on tarhana","DOI":null,"author":[{"given":"O.","family":"Gurbuz"},{"given":"D.","family":"Gocmen"},{"given":"N.","family":"Ozmen"},{"given":"F.","family":"Dagdelen"}],"issued":{"date-parts":["2010"]},"container-title":"Preparative Biochemistry and Biotechnology","volume":"40","issue":"4","page":"263\u2013275"}]

[{"id":2065,"citationNumber":"23.","type":"article-journal","title":"Chemical composition and antimicrobial activity of the essential oil of Ferulago bernardii Tomk","DOI":null,"author":[{"given":"F.","family":"Khalighi-Sigaroodi"},{"given":"A.","family":"Hadjiakhoondi"},{"given":"A.R.","family":"Shahverdi"},{"given":"V.-A.","family":"Mozaffarian"},{"given":"A.","family":"Shafiee\""}],"issued":{"date-parts":["2005"]},"container-title":"and M. Pimen\", DARU Journal of Pharmaceutical Sciences","volume":"13","issue":"3","page":"100\u2013104"}]

[{"id":2066,"citationNumber":"24.","type":"article-journal","title":"Inhibition of quorum sensing regulated bacterial functions by plant essential oils with special reference to clove oil","DOI":null,"author":[{"given":"M.","family":"Khan"},{"given":"M.","family":"Zahin"},{"given":"S.","family":"Hasan"},{"given":"F.","family":"Husain"},{"given":"I.","family":"Ahmad"}],"issued":{"date-parts":["2009"]},"container-title":"Letters in Applied Microbiology","volume":"49","issue":"3","page":"354\u2013360"}]

[{"id":2067,"citationNumber":"25.","type":"chapter","title":"","DOI":null,"author":[{"given":"K.","family":"Micevski"}],"issued":{"date-parts":["2005"]},"container-title":"Flora of Republic of Macedonia","volume":null,"issue":null,"page":"1543\u20131544"}]

[{"id":2068,"citationNumber":"26.","type":"article-journal","title":"Radical scavenging and antimicrobial activity of essential oil and extracts of Echinophora sibthorpiana Guss","DOI":null,"author":[{"given":"K.","family":"Mileski"},{"given":"A.","family":"D\u017eamic"},{"given":"A.","family":"Ciri\u0107"},{"given":"S.","family":"Gruji\u0107"},{"given":"V.","family":"Matevski"},{"given":"P.","family":"Marin"},{"given":"M.","family":"Risti\u0107"}],"issued":{"date-parts":["2014"]},"container-title":"from Macedonia, Archives of Biological Sciences","volume":"66","issue":"1","page":"401\u2013413"}]

[{"id":2069,"citationNumber":"27.","type":"article-journal","title":"Composition, antimicrobial and antioxidant properties of endemic species Ferulago macedonica Micevski & E","DOI":null,"author":[{"given":"K.","family":"Mileski"},{"given":"A.","family":"D\u017eamic"},{"given":"A.","family":"Ciri\u0107"},{"given":"M.","family":"Risti\u0107"},{"given":"S.","family":"Gruji\u0107"},{"given":"V.","family":"Matevski"},{"given":"P.","family":"Marin"}],"issued":{"date-parts":["2015"]},"container-title":"Mayer, Records of Natural Products","volume":"9","issue":"2","page":"208\u2013223"}]

[{"id":2070,"citationNumber":"28.","type":"article-journal","title":"Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis","DOI":null,"author":[{"given":"G.A.","family":"O\u2019Toole"},{"given":"R.","family":"Kolter"}],"issued":{"date-parts":["1998"]},"container-title":"Molecular Microbiology","volume":"28","issue":"3","page":"449\u2013461"}]

[{"id":2071,"citationNumber":"29.","type":"article-journal","title":"Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development","DOI":null,"author":[{"given":"G.A.","family":"OToole"},{"given":"R.","family":"Kolter"}],"issued":{"date-parts":["1998"]},"container-title":"Molecular Microbiology","volume":"30","issue":"2","page":"295\u2013304"}]

[{"id":2072,"citationNumber":"30.","type":"article-journal","title":"A Traditional Turkish Fermented Cereal Food: Tarhana","DOI":null,"author":[{"given":"S.","family":"Ozdemir"},{"given":"D.","family":"Gocmen"},{"given":"A.","family":"Yildirim Kumral"}],"issued":{"date-parts":["2007"]},"container-title":"Food Reviews International","volume":"23","issue":"2","page":"107\u2013121"}]

[{"id":2073,"citationNumber":"31.","type":"article-journal","title":"Composition and Antimicrobial Activity of Essential Oil of Ferulago longistylis Boiss","DOI":null,"author":[{"given":"A.M.G.","family":"Ozkan"},{"given":"B.","family":"Demirci"},{"given":"F.","family":"Demirci"},{"given":"K.H.C","family":"Baser"}],"issued":{"date-parts":["2008"]},"container-title":"Fruits, Journal of Essential Oil Research","volume":"20","issue":"6","page":"569\u2013573"}]

[{"id":2074,"citationNumber":"32.","type":"article-journal","title":"European Pharmacopoeia 8.0","DOI":null,"author":[{"given":"PhEur80","family":""}],"issued":{"date-parts":["2013"]},"container-title":"Council of Europe, Strasbourg","volume":null,"issue":null,"page":null}]

[{"id":2075,"citationNumber":"33.","type":"article-journal","title":"The investigation on phytochemicals from Ferulago angulata (Schlecht) Boiss, indigenous to central parts of Iran","DOI":null,"author":[{"given":"S.M.","family":"Razavi"},{"given":"A.","family":"Ravansalar"},{"given":"S.","family":"Mirinejad"}],"issued":{"date-parts":["2015"]},"container-title":"Natural Product Research","volume":"29","issue":"21","page":"2037\u20132040"}]

[{"id":2076,"citationNumber":"34.","type":"article-journal","title":"Chemical Constituents of Ferulago nodosa","DOI":null,"author":[{"given":"G.","family":"Ruberto"},{"given":"S.","family":"Cannizzo"},{"given":"V.","family":"Amico"},{"given":"M.","family":"Bizzini"},{"given":"M.","family":"Piattelli"}],"issued":{"date-parts":["1994"]},"container-title":"Journal of Natural Products","volume":"57","issue":"12","page":"1731\u20131733"}]

[{"id":2077,"citationNumber":"35.","type":"article-journal","title":"Chemical Composition and Antioxidative Activity of Echinophora platyloba DC. Essential Oil, and Its Interaction with Natural Antimicrobials against Food-Borne Pathogens and Spoilage Organisms","DOI":null,"author":[{"given":"S.S.","family":"Saei-Dehkordi"},{"given":"A.A.","family":"Fallah"},{"given":"S.S.","family":"Saei-Dehkordi"},{"given":"S.","family":"Kousha"}],"issued":{"date-parts":["2012"]},"container-title":"Journal of Food Science","volume":"77","issue":"11","page":"631\u2013 637"}]

[{"id":2078,"citationNumber":"36.","type":"article-journal","title":"Inhibition of quorum sensing in Pseudomonas aeruginosa by two herbal essential oils from Apiaceae family","DOI":null,"author":[{"given":"E.","family":"Sepahi"},{"given":"S.","family":"Tarighi"},{"given":"F.S.","family":"Ahmadi"},{"given":"A.","family":"Bagheri"}],"issued":{"date-parts":["2015"]},"container-title":"Journal of Microbiology","volume":"53","issue":"2","page":"176\u2013180"}]

[{"id":2079,"citationNumber":"37.","type":"article-journal","title":"Antioxidant and antibacterial activity of the extracts of Echinophora platyloba D.C","DOI":null,"author":[{"given":"R.","family":"Sharafati\u2013chaleshtori"},{"given":"M.","family":"Rafieian\u2013kopaei"},{"given":"S.","family":"Mortezaei"},{"given":"A.","family":"Sharafati\u2013chaleshtori"},{"given":"E.","family":"Amini"}],"issued":{"date-parts":["2012"]},"container-title":"African Journal of Pharmacy and Pharmacology","volume":"6","issue":null,"page":"2692\u20132695"}]

[{"id":2080,"citationNumber":"38.","type":"article-journal","title":"Agaricus Blazei Hot Water Extract Shows Anti Quorum Sensing Activity in the Nosocomial Human Pathogen Pseudomonas aeruginosa","DOI":null,"author":[{"given":"M.","family":"Sokovi\u0107"},{"given":"A.","family":"Ciri\u0107"},{"given":"J.","family":"Glamo\u0107lija"},{"given":"M.","family":"Nikoli\u0107"},{"given":"L.","family":"Griensven"}],"issued":{"date-parts":["2014"]},"container-title":"Molecules","volume":"19","issue":"4","page":"4189\u20134199"}]

[{"id":2081,"citationNumber":"39.","type":"article-journal","title":"Biofilms and Planktonic Cells of Pseudomonas aeruginosa Have Similar Resistance to Killing by Antimicrobials","DOI":null,"author":[{"given":"A.L.","family":"Spoering"},{"given":"K.","family":"Lewis"}],"issued":{"date-parts":["2001"]},"container-title":"Journal of Bacteriology","volume":"183","issue":"23","page":"6746\u20136751"}]

[{"id":2082,"citationNumber":"40.","type":"article-journal","title":"Antimicrobial activity of essential oils of Ferulago angulata subsp. carduchorum","DOI":null,"author":[{"given":"M.","family":"Taran"},{"given":"H.","family":"Reza Ghasempour"},{"given":"S.","family":"Ebrahim"}],"issued":{"date-parts":["2010"]},"container-title":"Jundishapur Journal of Microbiology","volume":"3","issue":null,"page":null}]

[{"id":2083,"citationNumber":"41.","type":"article-journal","title":"Plants Secrete Substances That Mimic Bacterial N -Acyl Homoserine Lactone Signal Activities and Affect Population DensityDependent Behaviors in Associated Bacteria","DOI":null,"author":[{"given":"M.","family":"Teplitski"},{"given":"J.B.","family":"Robinson"},{"given":"W.D.","family":"Bauer"}],"issued":{"date-parts":["2000"]},"container-title":"Molecular Plant-Microbe Interactions","volume":"13","issue":"6","page":"637\u2013648"}]

[{"id":2084,"citationNumber":"42.","type":"chapter","title":"","DOI":null,"author":[{"given":"T.","family":"Tutin"}],"issued":{"date-parts":["1968"]},"container-title":"Flora Europaea","volume":null,"issue":null,"page":"324"}]

[{"id":2085,"citationNumber":"43.","type":"article-journal","title":"Isolation and Structure Elucidation of Secondary Metabolites from Echinophora platyloba DC from Iran","DOI":null,"author":[{"given":"H.","family":"Valizadeh"},{"given":"F.","family":"Mahmoodi"},{"given":"Z.","family":"Alizadeh"},{"given":"M.B.","family":"Bahadori"}],"issued":{"date-parts":["2014"]},"container-title":"Journal of Medicinal Plants","volume":"13","issue":null,"page":"15\u201321"}]

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