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Research Article

Antioxidant and antineurodegenerative properties of St. John’s-wort dry extract

By
Katarina Šavikin ,
Katarina Šavikin

Institute for Medicinal Plant Research “Dr. Josif Panci ˇ c”, Belgrade, Serbia

Ana Alimpić ,
Ana Alimpić

Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Belgrade, Serbia

Gordana Zdunić ,
Gordana Zdunić

Institute for Medicinal Plant Research “Dr. Josif Panci ˇ c”, Belgrade, Serbia

Jelena Živković ,
Jelena Živković

Institute for Medicinal Plant Research “Dr. Josif Panci ˇ c”, Belgrade, Serbia

Teodora Janković ,
Teodora Janković

Institute for Medicinal Plant Research “Dr. Josif Pančić”, Belgrade, Serbia

Nebojša Menković ,
Nebojša Menković

Institute for Medicinal Plant Research “Dr. Josif Pančić”, Belgrade, Serbia

Sonja Duletić-Laušević
Sonja Duletić-Laušević

Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Belgrade, Serbia

Abstract

Different biological activities of Hypericum perforatum L. dry extract was tested in this study. Antioxidant activity was evaluated by four different assays. Antineurodegenerative activity was tested using acetylcholinesterase (AChE) and tyrosinase inhibitory activity assays. In all four antioxidant assays, concentration dependent manner was noticed in the activity of tested extract. The most active was the highest applied concentration (500 µg/ml). In DPPH test, extract applied in concentration of 200 µg/ml showed similar or higher radical scavenging activity compared to the concentration of 100 µg/ml applied for standard substances BHA and BHT. The lowest antioxidant activity for the extract was detected in ABTS test. All applied concentrations of dry extract were significantly more potent than vitamin C in β-carotene test. Moreover, the extract reached 40.31 and 45.56% of the inhibition in AChE and TYR assays, respectively. In both cases, the most active concentration of the extract was 200 µg/ml.

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