×
Home Current Archive Editorial board
News Contact
Originalan naučni rad– Original scientific paper

IN VITRO ANTIFUNGAL POTENTIAL OF BACILLUS SPP. ISOLATES AS BIOCONTROL AGENTS

By
Ivica Dikić ,
Ivica Dikić

Faculty of Biology, University of Belgrade, Belgrade, Serbia

Tatjana Stević ,
Tatjana Stević
Contact Tatjana Stević

Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia

Tanja Berić ,
Tanja Berić

Faculty of Biology, University of Belgrade, Belgrade, Serbia

Ivan Nikolić ,
Ivan Nikolić

Faculty of Biology, University of Belgrade, Belgrade, Serbia

Tamara Janakiev ,
Tamara Janakiev

Faculty of Biology, University of Belgrade, Belgrade, Serbia

Đorđe Fira ,
Đorđe Fira

Faculty of Biology, University of Belgrade, Belgrade, Serbia

Slaviša Stanković
Slaviša Stanković

Faculty of Biology, University of Belgrade, Belgrade, Serbia

Abstract

Plant diseases caused by infection with pathogenic fungi can lead to the reduction in the capacity of plant growth or can cause far more serious damage, leading to the death of plants and significant losses in food production. Numerous recent studies are devoted to the investigation of bacteria from genus Bacillus as producers of secondary metabolites that can be used in the control of different plant pathogens. In this study, strong antifungal effect of lipopeptide extracts towards 11 fungi tested was observed, with lowest recorded minimal inhibitory concentrations of 0.008 mg/ml against Fusarium semitectum. In the analysis of the interaction of lipopeptide extracts mutually, as well as in combination with essential oils, the existence of a synergistic effect in vitro was shown. In combination of isolate SS12.6 extract and savory oil on the growth of Alternaria alternata, as well as in combination of the same extract and thyme oil in acting against Fusarium nygamai, synergistic effect was achieved. The combination of both oils and extract of SS12.6 showed synergistic activity only against Fusarium solani. To date, it has been shown that the combination of complementary biological approaches with additive and/or synergistic effect may provide greater consistency and efficiency in biocontrol, so in that sense there is a growing interest for agents that could possible act as replacement of conventional synthetic fungicides in protection of cultivated plants from phytopathogenic fungi.


 

References

[{"id":1832,"citationNumber":"1.","type":"article-journal","title":"Biological control of plant pathogens","DOI":null,"author":[{"given":"K.","family":"Pal"},{"given":"M.","family":"Gardener"},{"given":"B.","family":"M"}],"issued":{"date-parts":["2006"]},"container-title":"The Plant Health Instructor","volume":"2","issue":null,"page":"1117\u20131142"}]

[{"id":1833,"citationNumber":"2.","type":"chapter","title":"Bacillus-based biological control of plant diseases. Pesticides in the modern world\u2014pesticides use and management","DOI":null,"author":[{"given":"H.","family":"Cawoy"},{"given":"W.","family":"Bettiol"},{"given":"P.","family":"Fickers"},{"given":"M.","family":"Ongena"}],"issued":{"date-parts":["2011"]},"container-title":"Tech","volume":null,"issue":null,"page":"273\u2013302"}]

[{"id":1834,"citationNumber":"3.","type":"article-journal","title":"Comparative genome analysis of Bacillus spp. and its relationship with bioactive nonribosomal peptide production","DOI":null,"author":[{"given":"A.","family":"Hamdache"},{"given":"R.","family":"Azarken"},{"given":"A.","family":"Lamarti"},{"given":"J.","family":"Aleu"},{"given":"G.","family":"Collado"}],"issued":{"date-parts":["2013"]},"container-title":"Phytochemistry Reviews","volume":"12","issue":"4","page":"685\u2013716"}]

[{"id":1835,"citationNumber":"4.","type":"article-journal","title":"Bacillus lipopeptides: versatile weapons for plant disease biocontrol","DOI":null,"author":[{"given":"M.","family":"Ongena"},{"given":"P.","family":"Jacques"}],"issued":{"date-parts":["2008"]},"container-title":"Trends in Microbiology","volume":"16","issue":"3","page":"115\u2013125"}]

[{"id":1836,"citationNumber":"5.","type":"article-journal","title":"Diversity of nonribosomal peptide synthetases involved in the biosynthesis of lipopeptide biosurfactants","DOI":null,"author":[{"given":"N.","family":"Roongsawang"},{"given":"K.","family":"Washio"},{"given":"M.","family":"Morikawa"}],"issued":{"date-parts":["2010"]},"container-title":"International Journal of Molecular Sciences","volume":"12","issue":"1","page":"141\u2013172"}]

[{"id":1837,"citationNumber":"6.","type":"article-journal","title":"Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14","DOI":null,"author":[{"given":"O.","family":"Asaka"},{"given":"M.","family":"Shoda"}],"issued":{"date-parts":["1996"]},"container-title":"Applied and Environmental Microbiology","volume":"62","issue":"11","page":"4081\u20134085"}]

[{"id":1838,"citationNumber":"7.","type":"article-journal","title":"Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the 177 organism's antagonistic and biocontrol activities","DOI":null,"author":[{"given":"V.","family":"Lecl\u00e8re"},{"given":"M.B\u00e9chet A.","family":"Adam"},{"given":"S.","family":"Guez"},{"given":"B.","family":"Wathelet"},{"given":"M.","family":"Ongena"},{"given":"P.","family":"Jacques"}],"issued":{"date-parts":["2005"]},"container-title":"Applied and Environmental Microbiology","volume":"71","issue":"8","page":"4577\u20134584"}]

[{"id":1839,"citationNumber":"8.","type":"article-journal","title":"The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca","DOI":null,"author":[{"given":"D.","family":"Romero"},{"given":"A.","family":"Vicente"},{"given":"H.","family":"Rakotoaly"},{"given":"E.","family":"Dufour"},{"given":"W.","family":"Veening"},{"given":"E.","family":"Arrebola"},{"given":"A.","family":"P\u00e9rez-Garc\u00eda"}],"issued":{"date-parts":["2007"]},"container-title":"Molecular Plant-Microbe Interactions","volume":"20","issue":"4","page":"430\u2013440"}]

[{"id":1840,"citationNumber":"9.","type":"article-journal","title":"Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple","DOI":null,"author":[{"given":"Y.","family":"Toure"},{"given":"M.","family":"Ongena"},{"given":"P.","family":"Jacques"},{"given":"A.","family":"Guiro"},{"given":"P.","family":"Thonart"}],"issued":{"date-parts":["2004"]},"container-title":"Journal of Applied Microbiology","volume":"96","issue":"5","page":"1151\u20131160"}]

[{"id":1841,"citationNumber":"10.","type":"article-journal","title":"Characterization of Fusarium graminearum inhibitory lipopeptide from Bacillus subtilis IB","DOI":null,"author":[{"given":"J.","family":"Wang"},{"given":"J.","family":"Liu"},{"given":"H.","family":"Chen"},{"given":"J.","family":"Yao"}],"issued":{"date-parts":["2007"]},"container-title":"Applied Microbiology and Biotechnology","volume":"76","issue":"4","page":"889\u2013894"}]

[{"id":1842,"citationNumber":"11.","type":"article-journal","title":"Mulberry anthracnose antagonists (iturins) produced by Bacillus amyloliquefaciens RC2","DOI":null,"author":[{"given":"S.","family":"Hiradate"},{"given":"S.","family":"Yoshida"},{"given":"H.","family":"Sugie"},{"given":"H.","family":"Yada"},{"given":"Y.","family":"Fujii"}],"issued":{"date-parts":["2002"]},"container-title":"Phytochemistry","volume":"61","issue":"6","page":"693\u2013698"}]

[{"id":1843,"citationNumber":"12.","type":"article-journal","title":"An antifungal compound produced by Bacillus subtilis YM 10\u201320 inhibits germination of Penicillium roqueforti conidiospores","DOI":null,"author":[{"given":"S.","family":"Chitarra"},{"given":"P.","family":"Breeuwer"},{"given":"R.","family":"Nout"},{"given":"C.","family":"Aelst"},{"given":"M.","family":"Rombouts"},{"given":"T.","family":"Abee"}],"issued":{"date-parts":["2003"]},"container-title":"Journal of Applied Microbiology","volume":"94","issue":"2","page":"159\u2013166"}]

[{"id":1844,"citationNumber":"13.","type":"article-journal","title":"Bacillomycin D: an iturin with antifungal activity against Aspergillus flavus","DOI":null,"author":[{"given":"L.","family":"Moyne"},{"given":"R.","family":"Shelby"},{"given":"E.","family":"Cleveland"},{"given":"S.","family":"Tuzun"}],"issued":{"date-parts":["2001"]},"container-title":"Journal of Applied Microbiology","volume":"90","issue":"4","page":"622\u2013629"}]

[{"id":1845,"citationNumber":"14.","type":"article-journal","title":"Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani","DOI":null,"author":[{"given":"Y.","family":"Yu"},{"given":"B.","family":"Sinclair"},{"given":"L.","family":"Hartman"},{"given":"L.","family":"Bertagnolli"}],"issued":{"date-parts":["2002"]},"container-title":"Soil Biology and Biochemistry","volume":"34","issue":"7","page":"955\u2013963"}]

[{"id":1846,"citationNumber":"15.","type":"article-journal","title":"Evaluation of antagonistic activities of Bacillus subtilis and Bacillus licheniformis against wood-staining fungi: in vitro and in vivo experiments","DOI":null,"author":[{"given":"N.","family":"Velmurugan"},{"given":"S.","family":"Choi"},{"given":"S.","family":"Han"},{"given":"S.","family":"Lee"}],"issued":{"date-parts":["2009"]},"container-title":"The Journal of Microbiology","volume":"47","issue":"4","page":"385\u2013 392"}]

[{"id":1847,"citationNumber":"16.","type":"article-journal","title":"Compounds inhibitory to nematophagous fungi produced by Bacillus sp. strain H6 isolated from fungistatic soil","DOI":null,"author":[{"given":"L.","family":"Li"},{"given":"M.","family":"Mo"},{"given":"Q.","family":"Qu"},{"given":"H.","family":"Luo"},{"given":"K.","family":"Zhang"}],"issued":{"date-parts":["2007"]},"container-title":"European Journal of Plant Pathology","volume":"117","issue":"4","page":"329\u2013340"}]

[{"id":1848,"citationNumber":"17.","type":"article-journal","title":"Effect of a highly concentrated lipopeptide extract of Bacillus subtilis on fungal and bacterial cells","DOI":null,"author":[{"given":"A.","family":"Etchegaray"},{"given":"C.","family":"Castro Bueno"},{"given":"S.","family":"Melo"},{"given":"M.","family":"Tsai"},{"given":"M.","family":"F\u00e1tima Fiore"},{"given":"E.","family":"Silva-Stenico"},{"given":"O.","family":"Teschke"}],"issued":{"date-parts":["2008"]},"container-title":"Archives of Microbiology","volume":"190","issue":"6","page":"611\u2013622"}]

[{"id":1849,"citationNumber":"18.","type":"article-journal","title":"Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi","DOI":null,"author":[{"given":"I.","family":"Dimki\u0107"},{"given":"S.","family":"\u017divkovi\u0107"},{"given":"T.","family":"Beri\u0107"},{"given":"\u017d.","family":"Ivanovi\u0107"},{"given":"V.","family":"Gavrilovi\u0107"},{"given":"S.","family":"Stankovi\u0107"},{"given":"D.","family":"Fira"}],"issued":{"date-parts":["2013"]},"container-title":"Biological Control","volume":"65","issue":"3","page":"312\u2013321 178"}]

[{"id":1850,"citationNumber":"19.","type":"article-journal","title":"Additive and synergistic effects of Bacillus spp. isolates and essential oils on the control of phytopathogenic and saprophytic fungi from medicinal plants and marigold seeds","DOI":null,"author":[{"given":"I.","family":"Dimki\u0107"},{"given":"T.","family":"Beri\u0107"},{"given":"T.","family":"Stevi\u0107"},{"given":"S.","family":"Pavlovi\u0107"},{"given":"K.\u0160avikin","family":"S."},{"given":"D.","family":"Fira"},{"given":"S.","family":"Stankovi\u0107"}],"issued":{"date-parts":["2015"]},"container-title":"Biological Control","volume":"87","issue":null,"page":"6\u201313"}]

[{"id":1851,"citationNumber":"20.","type":"article-journal","title":"Subspecies-specific distribution of intervening sequences in the Bacillus subtilis prophage ribonucleotide reductase genes","DOI":null,"author":[{"given":"S.","family":"Stankovic"},{"given":"B.","family":"Soldo"},{"given":"T.","family":"Beric-Bjedov"},{"given":"J.","family":"Knezevic-Vukcevic"},{"given":"D.","family":"Simic"},{"given":"V.","family":"Lazarevic"}],"issued":{"date-parts":["2007"]},"container-title":"Systematic and Applied Microbiology","volume":"30","issue":"1","page":"8\u201315"}]

[{"id":1852,"citationNumber":"21.","type":"article-journal","title":"Matrixassisted laser desorption ionization-time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge","DOI":null,"author":[{"given":"J.","family":"Vater"},{"given":"B.","family":"Kablitz"},{"given":"C.","family":"Wilde"},{"given":"P.","family":"Franke"},{"given":"N.","family":"Mehta"},{"given":"S.","family":"Cameotra"}],"issued":{"date-parts":["2002"]},"container-title":"Applied and Environmental Microbiology","volume":"68","issue":"12","page":"6210\u20136219"}]

[{"id":1853,"citationNumber":"22.","type":"chapter","title":"Fungal culture media","DOI":null,"author":[{"given":"C.","family":"Booth"}],"issued":{"date-parts":["1971"]},"container-title":"Methods in Microbiology","volume":null,"issue":null,"page":"49\u201394"}]

[{"id":1854,"citationNumber":"23.","type":"article-journal","title":"Terminology relating to methods for the determination of susceptibility of bacteria to antimicrobial agents","DOI":null,"author":[{"given":"European Committee for Antimicrobial Susceptibility Testing ","family":""}],"issued":{"date-parts":["2000"]},"container-title":"Clinical Microbiology and Infection","volume":"Infection,6","issue":null,"page":"503\u2013508"}]

[{"id":1855,"citationNumber":"24.","type":"article-journal","title":"Effects of essential oils on phytopathogenic fungi in vitro","DOI":null,"author":[{"given":"A.","family":"Zambonelli"},{"given":"Z.","family":"d'Aulerio"},{"given":"A.","family":"Bianchi"},{"given":"A.","family":"Albasini"}],"issued":{"date-parts":["1996"]},"container-title":"Journal of Phytopathology","volume":"144","issue":"9\u201010","page":"491\u2013494"}]

[{"id":1856,"citationNumber":"25.","type":"article-journal","title":"Antifungal activity of selected essential oils against fungi isolated from medicinal plant","DOI":null,"author":[{"given":"T.","family":"Stevi\u0107"},{"given":"Beri\u0107","family":"T."},{"given":"K.","family":"\u0160avikin"},{"given":"M.","family":"Sokovi\u0107"},{"given":"D.","family":"Go\u0111evac"},{"given":"I.","family":"Dimki\u0107"},{"given":"S.","family":"Stankovi\u0107"}],"issued":{"date-parts":["2014"]},"container-title":"Industrial Crops and Products","volume":"55","issue":null,"page":"116\u2013122"}]

[{"id":1857,"citationNumber":"26.","type":"article-journal","title":"Mode of action of Bacillus subtilis for control of avocado postharvest pathogens","DOI":null,"author":[{"given":"L.","family":"Korsten"},{"given":"S.","family":"Jager"}],"issued":{"date-parts":["1995"]},"container-title":"South African Avocado Growers' Association Yearbook","volume":"18","issue":null,"page":"124\u2013130"}]

[{"id":1858,"citationNumber":"27.","type":"article-journal","title":"Synergism: a patent view","DOI":null,"author":[{"given":"L.","family":"Richer"}],"issued":{"date-parts":["1987"]},"container-title":"Pest Management Science","volume":"19","issue":null,"page":"309\u2013 315"}]

[{"id":1859,"citationNumber":"28.","type":"article-journal","title":"Ecological and mechanistic insights into the direct and indirect antimicrobial properties of Bacillus subtilis lipopeptides on plant pathogens","DOI":null,"author":[{"given":"J.","family":"Falardeau"},{"given":"C.","family":"Wise"},{"given":"L.","family":"Novitsky"},{"given":"J.","family":"Avis"}],"issued":{"date-parts":["2013"]},"container-title":"Journal of Chemical Ecology","volume":"39","issue":"7","page":"869\u2013878"}]

[{"id":1860,"citationNumber":"29.","type":"article-journal","title":"Isolation and identification of lipopeptides produced by B. subtilis SQR 9 for suppressing Fusarium wilt of cucumber","DOI":null,"author":[{"given":"Y.","family":"Cao"},{"given":"Z.","family":"Xu"},{"given":"N.","family":"Ling"},{"given":"Y.","family":"Yuan"},{"given":"X.","family":"Yang"},{"given":"L.","family":"Chen"},{"given":"Q.","family":"Shen"}],"issued":{"date-parts":["2012"]},"container-title":"Scientia Horticulturae","volume":"135","issue":null,"page":"32\u201339"}]

[{"id":1861,"citationNumber":"30.","type":"article-journal","title":"Isolation and characterization of antagonistic Bacillus subtilis strains from the avocado rhizoplane displaying biocontrol activity","DOI":null,"author":[{"given":"M.","family":"Cazorla"},{"given":"D.","family":"Romero"},{"given":"A.","family":"P\u00e9rez\u2010Garc\u00eda"},{"given":"J.","family":"Lugtenberg"},{"given":"D.","family":"Vicente"},{"given":"G.","family":"Bloemberg"}],"issued":{"date-parts":["2007"]},"container-title":"Journal of Applied Microbiology","volume":"103","issue":"5","page":"1950\u20131959 179"}]

[{"id":1862,"citationNumber":"31.","type":"article-journal","title":"Antifungal lipopeptides from Bacillus amyloliquefaciens strain BO7","DOI":null,"author":[{"given":"A.","family":"Romano"},{"given":"D.","family":"Vitullo"},{"given":"A.","family":"Pietro"},{"given":"G.","family":"Lima"},{"given":"V.","family":"Lanzotti"}],"issued":{"date-parts":["2011"]},"container-title":"Journal of Natural Products","volume":"74","issue":"2","page":"145\u2013151"}]

[{"id":1863,"citationNumber":"32.","type":"article-journal","title":"Antifungal cyclic lipopeptides from Bacillus amyloliquefaciens strain BO5A","DOI":null,"author":[{"given":"A.","family":"Romano"},{"given":"D.","family":"Vitullo"},{"given":"M.","family":"Senatore"},{"given":"G.","family":"Lima"},{"given":"V.","family":"Lanzotti"}],"issued":{"date-parts":["2013"]},"container-title":"Journal of Natural Products","volume":"76","issue":"11","page":"2019\u20132025"}]

[{"id":1864,"citationNumber":"33.","type":"article-journal","title":"Antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against Rhizopus stolonifer","DOI":null,"author":[{"given":"Y.","family":"Tao"},{"given":"M.","family":"Bie"},{"given":"X.","family":"Lv"},{"given":"Z.","family":"Zhao"},{"given":"X.","family":"Lu"}],"issued":{"date-parts":["2011"]},"container-title":"The Journal of Microbiology","volume":"49","issue":"1","page":"146\u2013150"}]

[{"id":1865,"citationNumber":"34.","type":"article-journal","title":"Essential oils and Trichoderma harzianum as an integrated control measure against faba bean root rot pathogens","DOI":null,"author":[{"given":"M.","family":"Abdel-Kader"},{"given":"N.","family":"El-Mougy"},{"given":"S.","family":"Lashin"}],"issued":{"date-parts":["2011"]},"container-title":"Journal of Plant Protection Research","volume":"51","issue":"3","page":"306\u2013313"}]

[{"id":1866,"citationNumber":"35.","type":"article-journal","title":"Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases","DOI":null,"author":[{"given":"E.","family":"Arrebola"},{"given":"D.","family":"Sivakumar"},{"given":"R.","family":"Bacigalupo"},{"given":"L.","family":"Korsten"}],"issued":{"date-parts":["2010"]},"container-title":"Crop Protection","volume":"29","issue":"4","page":"369\u2013377"}]

[{"id":1867,"citationNumber":"36.","type":"article-journal","title":"Effect of essential plant oils on storage fungi, germination and emergence of cowpea seeds","DOI":null,"author":[{"given":"Q.","family":"Kritzinger"},{"given":"S.","family":"Aveling"},{"given":"O.","family":"Marasas"}],"issued":{"date-parts":["2002"]},"container-title":"Seed Science and","volume":null,"issue":"nology,30","page":"609\u2013619"}]

[{"id":1868,"citationNumber":"37.","type":"article-journal","title":"Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy","DOI":null,"author":[{"given":"F.","family":"Carson"},{"given":"J.","family":"Mee"},{"given":"V.","family":"Riley"}],"issued":{"date-parts":["2002"]},"container-title":"Antimicrobial Agents and Chemotherapy","volume":"46","issue":"6","page":"1914\u20131920"}]

Citation

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

Article metrics

Google scholar: See link

The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.