×
Home Current Archive Editorial board
News Contact
Originalan naučni rad– Original scientific paper

ANTIOXIDANT AND ANTIMICROBIAL POTENTIAL OF OPOPANAX HISPIDUS(APIACEAE) EXTRACTS

By
Jelena Matejić ,
Jelena Matejić

Faculty of Medicine, University of Nis, Niš, Serbia

Ana Džamić ,
Ana Džamić

Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Tatjana Mihajilov-Krstev ,
Tatjana Mihajilov-Krstev

Faculty of Science and Mathematics, University of Nis, Niš, Serbia

Vladimir Ranđelović ,
Vladimir Ranđelović

Faculty of Science and Mathematics, University of Nis, Niš, Serbia

Petar Marin
Petar Marin

Institute of Botany and Botanical Garden “Jevremovac”, Faculty of Biology, University of Belgrade, Belgrade, Serbia

Abstract

The present study was aimed to investigate antioxidant and antimicrobial potential of methanol and ethyl-acetate extracts from dried aerial parts, inflorescences and fruits of Opopanax hispidus (Friv.) Griseb., fam. Apiaceae. The antioxidant potential was evaluated with the help of two in vitro antioxidant models – DPPH and ABTS assays and estimation of total phenolic and flavonoids using spectrophotometric methods. BHA and Vitamin C were used as standard and positive control for above models. Microdilution assay was used to evaluate antimicrobial potential for the most common human gastrointestinal pathogenic microbial strains. The results of DPPH and ABTS assay showed that the highest antioxidant activity have methanol (IC50=1.157 mg/ml) and ethyl-acetate (IC50=3.167 mg/ml) extracts from inflorescences. The highest value of total phenolic (89.95±0.005 mg GA/g) and total flavonoid (24.06 ± 0.004 mg Qu/g) was measured in inflorescences extracts also. Results indicate that both extracts (methanol and ethyl-acetate) of inflorescences have high amount of phenol and flavonoids, which could be responsible for its good antioxidant activity.The most susceptible were Listeria monocytogenes and Escherichia coli on ethyl-acetate extracts from fruits and inflorescence, respectively. This is the first record of antioxidant and antimicrobial activity of Opopanax hispidus from Serbia. It is also worth noting that these results validate the therapeutic use of the plant in traditional medicine.


 

References

[{"id":1784,"citationNumber":"1.","type":"chapter","title":"In: Flora Europaea 2 (eds. T. G. Tutin, V. H. Heywood, N. A. Burges, D. M. Moore, D. H. Valentine, S. M. Walters, D. A. Webb)","DOI":null,"author":[{"given":"T.G.","family":"Tutin"}],"issued":{"date-parts":["1968"]},"container-title":"Cambridge University Press, London, UK","volume":null,"issue":null,"page":"360"}]

[{"id":1785,"citationNumber":"2.","type":"chapter","title":"In: The Red data book of flora of Serbia. Extinct and critically endangered taxa","DOI":null,"author":[{"given":"N.","family":"Randjelovi\u0107"},{"given":"V.","family":"Randjelovi\u0107"}],"issued":{"date-parts":["1999"]},"container-title":"Ministry of Environment of the Republic of Serbia, Faculty of Biology, University of Belgrade, Institution for Protection of Nature of the Republic of Serbia","volume":null,"issue":null,"page":null}]

[{"id":1786,"citationNumber":"3.","type":"article-journal","title":"The Essential Oils","DOI":null,"author":[{"given":"E.","family":"Guenther"}],"issued":{"date-parts":["1950"]},"container-title":", D. van Nostrand, New York","volume":"4","issue":null,"page":"349\u2013352,"}]

[{"id":1787,"citationNumber":"4.","type":"article-journal","title":"Die Harze","DOI":null,"author":[{"given":"O.","family":"Tschirch"},{"given":"E.","family":"Stock"}],"issued":{"date-parts":["1933"]},"container-title":"Borntraeger, Berlin","volume":"2","issue":null,"page":"184\u2013187,"}]

[{"id":1788,"citationNumber":"5.","type":"article-journal","title":"Coumarins from Opopanax chironium. New dihydrofuranocoumarins and differential induction of apoptosis by imperatorin and heraclenin","DOI":null,"author":[{"given":"G.","family":"Appendino"},{"given":"F.","family":"Bianchi"},{"given":"A.","family":"Bade"},{"given":"C.","family":"Campagnuolo"},{"given":"E.","family":"Fattorusso"},{"given":"O.","family":"Taglialatela-Scafati"},{"given":"M.","family":"Blanco-Molina"},{"given":"A.","family":"Macho"},{"given":"B.L.","family":"Fiebich"},{"given":"P.","family":"Bremner"},{"given":"M.","family":"Heinrich"},{"given":"M.","family":"Ballero"},{"given":"E.","family":"Mu\u00f1oz"}],"issued":{"date-parts":["2004"]},"container-title":"J. Nat. Prod","volume":"67","issue":null,"page":"532\u2013536"}]

[{"id":1789,"citationNumber":"6.","type":"article-journal","title":"The action of essential oils on phytopathogenic fungi","DOI":null,"author":[{"given":"J.C.","family":"Maruzzella"},{"given":"J.","family":"Balter"}],"issued":{"date-parts":["1959"]},"container-title":"Plant Dis. Rep","volume":"43","issue":null,"page":"1143\u20131147"}]

[{"id":1790,"citationNumber":"7.","type":"article-journal","title":"A new dihydrofuranocoumarin from Opopanaxhispidus (Friv","DOI":null,"author":[{"given":"S.","family":"Ghasemia"},{"given":"Z.","family":"Habibia"}],"issued":{"date-parts":["2014"]},"container-title":"Griseb. Nat. Prod. Res","volume":"28","issue":"21","page":"1808\u20131812"}]

[{"id":1791,"citationNumber":"8.","type":"article-journal","title":"Folk medicines in the villages of Il\u0131ca District (Erzurum, Turkey","DOI":null,"author":[{"given":"U.","family":"\u00d6zgen"},{"given":"Y.","family":"Kaya"},{"given":"P.","family":"Houghton"}],"issued":{"date-parts":["2012"]},"container-title":"Turk. J. Biol","volume":"36","issue":null,"page":"93\u2013106"}]

[{"id":1792,"citationNumber":"9.","type":"article-journal","title":"Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin\u2013Ciocalteu reagent","DOI":null,"author":[{"given":"V.L.","family":"Singleton"},{"given":"R.","family":"Orthofer"},{"given":"R.M.","family":"Lamuela-Raventos"}],"issued":{"date-parts":["1999"]},"container-title":"Meth. Enzymol","volume":"299","issue":null,"page":"152\u2013178"}]

[{"id":1793,"citationNumber":"10.","type":"article-journal","title":"Analysis of propolis: some parameters and procedures for chemical quality control","DOI":null,"author":[{"given":"R.","family":"Woisky"},{"given":"A.","family":"Salatino"}],"issued":{"date-parts":["1998"]},"container-title":"J. Apic. Res","volume":"37","issue":null,"page":"99\u2013105"}]

[{"id":1794,"citationNumber":"11.","type":"article-journal","title":"Antioxidant determinations by the use of a stable free radical","DOI":null,"author":[{"given":"M.S.","family":"Blois"}],"issued":{"date-parts":["1958"]},"container-title":"Nature","volume":"181","issue":null,"page":"1199\u20131200"}]

[{"id":1795,"citationNumber":"12.","type":"article-journal","title":"Factors influencing the antioxidant activity determined by the ABTS radical cation assay","DOI":null,"author":[{"given":"N.","family":"Miller"},{"given":"C.","family":"Rice-Evans"}],"issued":{"date-parts":["1997"]},"container-title":"Free Radic. Res","volume":"26","issue":null,"page":"195\u2013199 149"}]

[{"id":1796,"citationNumber":"13.","type":"article-journal","title":"National Committee for Clinical Laboratory Standards (2003): Performance standards for anti-microbial susceptibility testing: eleventh informational supplement","DOI":null,"author":[{"given":"N.C.C.L.S.","family":""}],"issued":{"date-parts":["2003"]},"container-title":"Document M100-S11, National Committee for Clinical Laboratory Standard, Wayne, PA, USA","volume":null,"issue":null,"page":null}]

[{"id":1797,"citationNumber":"14.","type":"article-journal","title":"Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil","DOI":null,"author":[{"given":"A.L.M.Machado","family":"Sartoratto"},{"given":"C.","family":"Delarmelina"},{"given":"G.M.","family":"Figueira"},{"given":"M.C.T.","family":"Duarte"},{"given":"V.L.G.","family":"Rehder"}],"issued":{"date-parts":["2004"]},"container-title":"Braz. J","volume":"Microbiol,35","issue":null,"page":"275\u2013 280"}]

[{"id":1798,"citationNumber":"15.","type":"article-journal","title":"Phenolic antioxidants","DOI":null,"author":[{"given":"F.","family":"Shahidi"},{"given":"P.K.J.P.D.","family":"Wanasundara"}],"issued":{"date-parts":["1992"]},"container-title":"Crit. Rev. Food Sci. Nutr","volume":"32","issue":null,"page":"67\u2013103"}]

[{"id":1799,"citationNumber":"16.","type":"article-journal","title":"Anti- and pro-oxidant activity of rutin and quercetin derivatives","DOI":null,"author":[{"given":"M.","family":"Kessler"},{"given":"G.","family":"Ubeaud"},{"given":"L.","family":"Jung"}],"issued":{"date-parts":["2003"]},"container-title":"J. Pharm Pharmacol","volume":"55","issue":null,"page":"131\u2013142"}]

[{"id":1800,"citationNumber":"17.","type":"article-journal","title":"Flavonoids- chemistry, metabolism, cardioprotective effects, and dietary sources","DOI":null,"author":[{"given":"N.C.","family":"Cook"},{"given":"S.","family":"Samman"}],"issued":{"date-parts":["1996"]},"container-title":"J. Nutr. Biochem","volume":"7","issue":null,"page":"66\u2013 76"}]

[{"id":1801,"citationNumber":"18.","type":"article-journal","title":"Tannins of tamaricaceeous plants. II- New dimeric hydrolysable tannins from Reaumuria hirtella","DOI":null,"author":[{"given":"T.","family":"Yoshida"},{"given":"A.F.","family":"Ahmed"},{"given":"T.","family":"Okuda"}],"issued":{"date-parts":["2007"]},"container-title":"Chem. Pharma. Bull","volume":"41","issue":null,"page":"672\u2013679"}]

[{"id":1802,"citationNumber":"19.","type":"article-journal","title":"Antioxidative activity of sixty plants from Iran","DOI":null,"author":[{"given":"E.","family":"Souri"},{"given":"G.","family":"Amin"},{"given":"A.D.","family":"Sherifabadi"},{"given":"A.","family":"Nazifi"},{"given":"H.","family":"Farsam"}],"issued":{"date-parts":["2004"]},"container-title":"J. Pharmac Res","volume":"3","issue":null,"page":"55\u201359"}]

[{"id":1803,"citationNumber":"20.","type":"article-journal","title":"Antibacterial activity of some plants from family Apiaceae in relation to selected phytopathogenic bacteria","DOI":null,"author":[{"given":"D.L.","family":"Brkovi\u0107"},{"given":"S.Soluji\u0107-Sukdolak","family":"Lj. \u010comi\u0107"}],"issued":{"date-parts":["2006"]},"container-title":"Kragujevac J. Sci","volume":"28","issue":null,"page":"65\u201372"}]

[{"id":1804,"citationNumber":"21.","type":"article-journal","title":"Antibacterial activity of some plants from the family Apiaceae growing wild in Serbia","DOI":null,"author":[{"given":"O.","family":"Stefanovic"},{"given":"D.","family":"Stanojevic"},{"given":"L.","family":"Comic"},{"given":"D.","family":"Brkovic"}],"issued":{"date-parts":["2007"]},"container-title":"Planta Med","volume":"73","issue":null,"page":"217"}]

[{"id":1805,"citationNumber":"22.","type":"article-journal","title":"Antioxidant and antimicrobial activities of extracts of four Peucedanum L. species","DOI":null,"author":[{"given":"J.S.","family":"Mateji\u0107"},{"given":"A.M.","family":"D\u017eami\u0107"},{"given":"A.D.","family":"\u0106iri\u0107"},{"given":"Z.","family":"Krivo\u0161ej"},{"given":"V.N.","family":"Ran\u0111elovi\u0107"},{"given":"P.D.","family":"Marin"}],"issued":{"date-parts":["2013"]},"container-title":"Dig. J. Nanomater. Bios","volume":"8","issue":"2","page":"655\u2013665"}]

[{"id":1806,"citationNumber":"23.","type":"article-journal","title":"Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pan\u010di\u0107 and Vis","DOI":null,"author":[{"given":"M.D.","family":"Mar\u010deti\u0107"},{"given":"S.D.","family":"Petrovi\u0107"},{"given":"M.T.","family":"Milenkovi\u0107"},{"given":"M.S.","family":"Niketi\u0107"}],"issued":{"date-parts":["2014"]},"container-title":"Apiaceae). Cent. Eur. J. Biol","volume":"9","issue":"2","page":"149\u2013155"}]

Citation

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 

Article metrics

Google scholar: See link

The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.