The present study was aimed to investigate antioxidant and antimicrobial potential of methanol and ethyl-acetate extracts from dried aerial parts, inflorescences and fruits of Opopanax hispidus (Friv.) Griseb., fam. Apiaceae. The antioxidant potential was evaluated with the help of two in vitro antioxidant models – DPPH and ABTS assays and estimation of total phenolic and flavonoids using spectrophotometric methods. BHA and Vitamin C were used as standard and positive control for above models. Microdilution assay was used to evaluate antimicrobial potential for the most common human gastrointestinal pathogenic microbial strains. The results of DPPH and ABTS assay showed that the highest antioxidant activity have methanol (IC50=1.157 mg/ml) and ethyl-acetate (IC50=3.167 mg/ml) extracts from inflorescences. The highest value of total phenolic (89.95±0.005 mg GA/g) and total flavonoid (24.06 ± 0.004 mg Qu/g) was measured in inflorescences extracts also. Results indicate that both extracts (methanol and ethyl-acetate) of inflorescences have high amount of phenol and flavonoids, which could be responsible for its good antioxidant activity.The most susceptible were Listeria monocytogenes and Escherichia coli on ethyl-acetate extracts from fruits and inflorescence, respectively. This is the first record of antioxidant and antimicrobial activity of Opopanax hispidus from Serbia. It is also worth noting that these results validate the therapeutic use of the plant in traditional medicine.
Appendino, G., Bianchi, F., Bade, A., Campagnuolo, C., Fattorusso, E., Taglialatela-Scafati, O., Blanco-Molina, M., Macho, A., Fiebich, B. L., Bremner, P., Heinrich, M., Ballero, M., & Muñoz, E. (2004). Coumarins from Opopanax chironium. New dihydrofuranocoumarins and differential induction of apoptosis by imperatorin and heraclenin. J. Nat. Prod, 67, 532–536.
Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199–1200.
Brković, D. L., & Lj. Čomić, S. S.-S. (2006). Antibacterial activity of some plants from family Apiaceae in relation to selected phytopathogenic bacteria. Kragujevac J. Sci, 28, 65–72.
Cook, N. C., & Samman, S. (1996). Flavonoids- chemistry, metabolism, cardioprotective effects, and dietary sources. J. Nutr. Biochem, 7, 66–76.
Ghasemia, S., & Habibia, Z. (2014). A new dihydrofuranocoumarin from Opopanaxhispidus (Friv. Griseb. Nat. Prod. Res, 28(21), 1808–1812.
Guenther, E. (1950). The Essential Oils. , D. van Nostrand, New York, 4, 349-352,.
Kessler, M., Ubeaud, G., & Jung, L. (2003). Anti- and pro-oxidant activity of rutin and quercetin derivatives. J. Pharm Pharmacol, 55, 131–142.
Marčetić, M. D., Petrović, S. D., Milenković, M. T., & Niketić, M. S. (2014). Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. Apiaceae). Cent. Eur. J. Biol, 9(2), 149–155.
Maruzzella, J. C., & Balter, J. (1959). The action of essential oils on phytopathogenic fungi. Plant Dis. Rep, 43, 1143–1147.
Matejić, J. S., Džamić, A. M., Ćirić, A. D., Krivošej, Z., Ranđelović, V. N., & Marin, P. D. (2013). Antioxidant and antimicrobial activities of extracts of four Peucedanum L. species. Dig. J. Nanomater. Bios, 8(2), 655–665.
Miller, N., & Rice-Evans, C. (1997). Factors influencing the antioxidant activity determined by the ABTS radical cation assay. Free Radic. Res, 26, 195-199 149.
N.C.C.L.S. (2003). National Committee for Clinical Laboratory Standards (2003): Performance standards for anti-microbial susceptibility testing: eleventh informational supplement. Document M100-S11, National Committee for Clinical Laboratory Standard, Wayne, PA, USA.
Özgen, U., Kaya, Y., & Houghton, P. (2012). Folk medicines in the villages of Ilıca District (Erzurum, Turkey. Turk. J. Biol, 36, 93–106.
Randjelović, N., & Randjelović, V. (1999). In: The Red data book of flora of Serbia. Extinct and critically endangered taxa. In Ministry of Environment of the Republic of Serbia, Faculty of Biology, University of Belgrade, Institution for Protection of Nature of the Republic of Serbia.
Sartoratto, A. L. M. M., Delarmelina, C., Figueira, G. M., Duarte, M. C. T., & Rehder, V. L. G. (2004). Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Braz. J, Microbiol,35, 275–280.
Shahidi, F., & Wanasundara, P. K. J. P. D. (1992). Phenolic antioxidants. Crit. Rev. Food Sci. Nutr, 32, 67–103.
Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Meth. Enzymol, 299, 152–178.
Souri, E., Amin, G., Sherifabadi, A. D., Nazifi, A., & Farsam, H. (2004). Antioxidative activity of sixty plants from Iran. J. Pharmac Res, 3, 55–59.
Stefanovic, O., Stanojevic, D., Comic, L., & Brkovic, D. (2007). Antibacterial activity of some plants from the family Apiaceae growing wild in Serbia. Planta Med, 73, 217.
Tschirch, O., & Stock, E. (1933). Die Harze. Borntraeger, Berlin, 2, 184-187,.
Tutin, T. G. (1968). In: Flora Europaea 2 (eds. T. G. Tutin, V. H. Heywood, N. A. Burges, D. M. Moore, D. H. Valentine, S. M. Walters, D. A. Webb). In Cambridge University Press, London, UK (p. 360).
Woisky, R., & Salatino, A. (1998). Analysis of propolis: some parameters and procedures for chemical quality control. J. Apic. Res, 37, 99–105.
Yoshida, T., Ahmed, A. F., & Okuda, T. (2007). Tannins of tamaricaceeous plants. II- New dimeric hydrolysable tannins from Reaumuria hirtella. Chem. Pharma. Bull, 41, 672–679.
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